基于GEO数据库对亚急性皮肤型红斑狼疮差异基因的筛选及生物信息学分析  

Screening and bioinformatics analysis of differential genes in subacute cutaneous lupus erythematosus based on GEO database

作　　者：王晓龙 杜金锋 赵金涛 裴广德 WANG Xiao-long;DU Jin-feng;ZHAO Jin-tao;PEI Guang-de(Department of Dermatology,Central Hospital of Jiaozuo Coal Group Company Ltd.,Jiaozuo,Henan 454000,China)

机构地区：[1]焦作煤业(集团)有限责任公司中央医院皮肤科,河南焦作454000

出　　处：《中国卫生检验杂志》2022年第21期2640-2643,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的运用生物信息学方法探讨亚急性皮肤型红斑狼疮(subacute cutaneous lupus erythematosis,SCLE)组织中的差异表达基因(differentially expressed genes,DEGs)。方法从GEO数据库下载数据集并筛选出SCLE组织中的DEGs,利用GO和KEGG分析对DEGs进行功能和通路注释,同时使用String数据库构建互作网络并筛选出Hub基因,选择和SCLE显著相关的前10个Hub基因并进行验证。结果对芯片数据集GSE112943进行DEGs分析后共得到显著上调的基因175个,显著下调的基因167个。GO富集分析DEGs主要富集于对病毒的防御反应、免疫反应、病毒基因组复制的负调控、I型干扰素信号通路及炎症反应等生物学进程,KEGG通路富集分析DEGs主要富集于细胞因子-细胞因子受体相互作用、细胞黏附分子、氨基酸代谢、细胞外基质和受体相互作用等通路。PPI网络中ISG15、RSAD2、IFIT3、IRF7、IFI44、IFI6、IFI44L、OAS1、OAS2和OAS3是SCLE的关键基因。相对于正常皮肤组织,SCLE患者皮损中ISG15、RSAD2、IFIT3、IRF7、IFI44、IFI6、IFI44L、OAS1、OAS2、OAS3表达水平升高,差异均有统计学意义(P<0.05)。结论SCLE的发病机制涉及到多种生物学过程及多种信号通路,ISG15、RSAD2、IFIT3等DEGs可能参与了SCLE的发生发展。Objective To investigate the differentially expressed genes(DEGs)in subacute cutaneous lupus erythematosis(SCLE)tissues using bioinformatics methods.Methods Data sets were download from GEO database and DEGs were screened out in SCLE organization.GO and KEGG analysis were used to annotate the functions and pathways of DEGs.At the same time,String database was used to construct interaction network and screen Hub genes.The top 10 Hub genes significantly related to SCLE were selected and verified.Results A total of 175 significantly up-regulated genes and 167 significantly down-regulated genes were obtained after DEGs analysis on the chip data set GSE112943.The results of GO enrichment analysis indicated that DEGs are mainly enriched in virus defense response,immune response,negative regulation of viral genome replication,type I interferon signaling pathway and inflammatory response and other biological processes.The results of KEGG pathway enrichment analysis indicated that DEGs are mainly enriched in cytokine-cytokine receptor interaction,cell adhesion molecules,amino acid metabolism,extracellular matrix and receptor interaction pathways.In the PPI results,ISG15,RSAD2,IFIT3,IRF7,IFI44,IFI6,IFI44L,OAS1,OAS2 and OAS3 are the hub genes of SCLE.Compared with normal skin tissue,the expression levels of ISG15,RSAD2,IFIT3,IRF7,IFI44,IFI6,IFI44L,OAS1,OAS2,OAS3 in the lesions of SCLE were increased,and the differences were statistically significant(P<0.05).Conclusion The pathogenesis of SCLE involves a variety of biological processes and multiple signaling pathways.DEGs such as ISG15,RSAD2,IFIT3 may be involved in the occurrence and development of SCLE.

关 键 词：亚急性皮肤型红斑狼疮 GEO数据库 生物学过程 发病机制 富集分析 

分 类 号：R758.62[医药卫生—皮肤病学与性病学]