青风藤及其混淆品种的生药学研究  

Pharmacognosy research on Sinomenii Caulis and its adulterants

作　　者：王新 韩文凯 SUTCHARITCHAN Chayanis 崔亚君[1] 曾茜垚 WANG Xin;HAN Wenkai;SUTCHARITCHAN Chayanis;CUI Yajun;ZENG Xiyao(School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Hunan Zhengqing Pharmaceutical Group Co.LTD,Huaihua 418000,Hunan,China)

机构地区：[1]上海中医药大学中药学院,上海201203 [2]湖南正清制药集团股份有限公司,湖南怀化418000

出　　处：《上海中医药大学学报》2022年第6期13-21,29,共10页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家重点研发计划中医药现代化研究专项“中药国际标准示范研究”项目(2018YFC1707900)。

摘　　要：目的:从性状鉴别、显微鉴别方面对青风藤及4种混淆品进行生药学研究,为解决青风藤药材品种混乱等问题提供参考依据。方法:采用光学显微镜和体视镜等仪器设备,对25个批次青风藤及4种混淆品进行系统的性状、微性状、显微正常光与偏振光对比观察研究,利用大型图像拼接技术和实时景深扩展技术采集全息彩色影像数据,从宏观到微观整体研究青风藤及其混淆品的形态学鉴别特征。结果:获取了青风藤及其混淆品性状、正常光和偏振光下组织横切面及粉末全息彩色显微影像图;建立了青风藤及混淆品性状、显微横切面及粉末快速鉴别检索表;通过组织横切面皮层、韧皮部组织的草酸钙结晶类型及分布规律可以快速区分青风藤及其混淆品。结论:偏振光下青风藤及其混淆品横切面全息彩色影像可进行清晰鉴别;偏振光下青风藤及其混淆品粉末可依据草酸钙结晶有无及存在形式、石细胞的有无快速鉴别。Objective:To conduct the pharmacognosy research on Sinomenii Caulis,and its four adulterants from the aspects of morphological identification and microscopic identification to provide reference for solving the problem of variety confusion of Sinomenii Caulis.Method:Optical microscope,stereoscope and other equipments were used to systematically observe and compare the traits,micro traits,normal light microscopic identification and polarized light microscopic identification of 25 batches Caulis Sinomenii and its four adulterants. Holographic color image data were collected by large image stitching technology and real-time depth of field expansion technology,and the morphological identification characteristics of Sinomenii Caulis and its adulterants were studied entirely from marcocosm to microcosm.Result:The traits of Sinomenii Caulis and its adulterants and the holographic color images of their tissue crosssections and powders under normal light microscope and polarized light microscope were obtained. An identification key was created for rapid identification on traits,microscopic cross-sections and powders of Sinomenii Caulis and its adulterants. Sinomenii Caulis and its adulterants can be quickly distinguished by the types and distribution patterns of calcium oxalate crystals in their cross-sectional cortex and phloem tissue.Conclusion:The holographic color images of the cross-section of Sinomenii Caulis can clearly identify Sinomenii Caulis and its adulterants under polarized light. Whether calcium oxalate crystals exist and their existence forms,and whether sclereid exists under polarized light can be used to quickly distinguish powder of Caulis Sinomenii and its adulterants.

关 键 词：青风藤 生药学 性状 显微 偏振光 

分 类 号：R282.5[医药卫生—中药学]