蜥蜴目同工酶表型效应与目内物种及种群间亲缘关系的研究  

Studies of the Phenotypic Character and Consanguinityof Isozymes in Species and Population

作　　者：韩雅莉[1] 肖湘[1] James Lazell 卢文华[2] 

机构地区：[1]汕头大学理学院生物系,广东汕头515063 [2]哈佛大学比较动物博物馆爬行动物系

出　　处：《遗传》2002年第6期670-674,共5页Hereditas（Beijing)

基　　金：汕头大学李嘉诚基金会人才基金(130-512044)资助

摘　　要：采用聚丙烯酰胺垂直板凝胶电泳技术和特异性的组织化学染色方法对分布于香港的Gekkochinensis、广东南澳岛和四川成都地区的Gekkosubpalmatus2个种群、以及香港和南澳岛的Hemidactylusbowringii2个种群蜥蜴目动物肝脏、骨骼肌的乳酸脱氢酶(LDH)和酯酶(EST)进行电泳分析。研究结果表明:属内种间具有明显的酶谱差异,每个种都有其特征酶带,据酶谱排序结果可以将5个种群分为2个类群,其中Hemidactylusbowringii的2个种群为一类群,Gekkochinensis和Gekkosubpalmatus的2个种群为另一类群。Using discontinuous polyacrylamide gel electrophoresis and histochemistry methods,lactate and esterase isozyme of three species and five popalation of Lacertiformes were analysed.The interspecific zymogramatic differences are obvious.Each species possesses its specific zymogram.According to the zymogramatic similarity among these species calculated with the method of polar ordination,they can be grouped into 2 categories.Gekko chinensis and the two population of Gekko subpalmatus fall into one category.The second category contains the two population of Hemidactylus bowringii.The congsanguinity of the species and popalation has been drawn from the results.

关 键 词：蜥蜴目 同工酶 表型效应 目内物种 种群间 亲缘关系 

分 类 号：Q753[生物学—分子生物学]