基于UPLC-Q-TOF/MS技术分析大高良姜醇提物的化学成分及胃溃疡寒证大鼠口服后的入血成分  

Chemical Profiling of Dagaoliangjiang(Alpinia galangal L.) Alcohol Extract and Blood Components of Rats with Gastric Ulcer with Cold Syndrome after Oral Administration Based on HPLC-Q-TOF/MS

作　　者：尹优 何瑞坤 黄焕迪 戴庆玲 钟贵 苏日香 秦华珍[1] YIN You;HE Ruikun;HUANG Huandi;DAI Qingling;ZHONG Gui;SU Rixiang;QIN Huazhen(Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China)

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《中华中医药学刊》2022年第11期142-147,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81760720);广西一流学科建设项目(2019XK073,2019XK069)。

摘　　要：目的 采用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF/MS)法分析大高良姜醇提物的主要化学成分及胃溃疡寒证大鼠口服后的入血成分。方法 回流提取法提取药物;大鼠灌胃醇提物后腹主动脉取血;分析采用Acquity HSS T3 C_(18)色谱柱(100 mm×2.1 mm, 1.8μm);流动相0.1%甲酸水-甲醇;梯度洗脱,流速0.4 mL·min^(-1);柱温35℃,MSE扫描正负离子模式检测。结果 综合色谱-质谱检测信息,包括色谱保留时间、分子量、离子碎片,结合相关文献报道,从大高良姜乙醇提取物中鉴定出17个化学成分;胃溃疡寒证大鼠口服大高良姜醇提物后,血清样本共鉴定出6个入血成分。结论 研究结果初步阐释了大高良姜的药效物质基础,为研究大高良姜抗胃溃疡寒证的药效物质提供了一定依据。Objective The chemical constituents of Dagaoliangjiang(Alpinia galangal L.)alcohol extract and the blood components of rats with gastric ulcer with cold syndrome after oral administration were analyzed by UPLC-Q-TOF/MS.Methods Reflux extraction method was used to extract drugs.The blood was collected from abdominal aorta of rats after intragastric administration of alcohol extract.Acquity HSS T3 C18 column(100 mm×2.1 mm,1.8μm)was used for analysis.Mobile phase was 0.1%formic acid water-methanol.The gradient elution rate was 0.4 mL·min-1.The column temperature was 35℃,and positive and negative ion modes were detected by MSE scanning.Results Based on the chromatography-mass spectrometry analysis,including chromatographic retention time,molecular weight,ion fragments and related literature reports,17 chemical constituents were identified from the ethanol extract of Dagaoliangjiang(Alpinia galangal L.).After oral administration of alcohol extract of Dagaoliangjiang(Alpinia galangal L.)in rats with gastric ulcer with cold syndrome,a total of 6 components were identified in serum samples.Conclusion The above research results preliminarily explained the pharmacological substance basis of Dagaoliangjiang(Alpinia galangal L.),and provided a certain basis for the study of the pharmacological substance of Dagaoliangjiang(Alpinia galangal L.)against cold syndrome of gastric ulcer.

关 键 词：大高良姜 UPLC-Q-TOF/MS 入血成分 

分 类 号：R284.1[医药卫生—中药学]