针刀疗法对兔退变颈椎间盘组织中p38MAPK蛋白与IL-1表达的影响  

Effects of Needle Knife Therapy on Expressions of p38MAPK Protein and IL-1in Rabbit Degenerative Cervical Disc Tissues

作　　者：李沅骋 李开平[1] 宋子琪 万成雨 张雅雯 李姜源 LI Yuancheng;LI Kaiping;SONG Ziqi;WANG Chengyu;ZHANG Yawen;LI Jiangyuan(School of Acupuncture and Tuina/Regimen and Rehabilitation College,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu,China)

机构地区：[1]南京中医药大学针灸推拿养生康复学院,江苏南京210023

出　　处：《中华中医药学刊》2022年第11期250-254,I0048,I0049,I0050,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省普通高校自然科学研究重大项目(18KJA360003);南京中医药大学教学改革研究开放项目;南京中医药大学中医学优势学科教学改革研究项目(ZYX03JG024)。

摘　　要：目的 观察针刀疗法对兔退变颈椎间盘组织中p38丝裂原活化蛋白激酶(p38MAPK)蛋白与白细胞介素-1(IL-1)表达的影响,探讨针刀治疗颈椎间盘退变的作用机制。方法 选用24只普通级的6月龄健康雄性新西兰大白兔,随机分为空白组、模型组与针刀组,每组8只。模型组与针刀组采用自制兔盒制作颈椎间盘退变模型。造模4周后,通过X线与MRI检查评价造模成功与否。造模结束后7 d,空白组与模型组不予任何干预措施;针刀组予以针刀干预,每周1次,共3次。干预结束后7 d,每组随机抽取2只行MRI检查,观察影像学变化。影像学检查结束后行空气栓塞法处死,立即取出椎间盘组织。HE染色观察其组织病理学变化,Western blot法检测p38MAPK的蛋白表达水平,免疫组织化学染色法检测IL-1的表达水平。结果 磁共振成像结果显示空白组见椎间盘高度正常,髓核呈亮白色,未见椎间隙变窄、椎间盘突出,纤维环边缘完整;模型组见椎间盘高度降低,髓核呈灰黑色,椎间隙变窄,C6/7椎间盘向后方突出,纤维环破裂;针刀组椎间盘高度正常,髓核呈灰色,未见椎间隙变窄,C6/7椎间盘向后方轻微突出,纤维环边缘尚完整。HE染色结果见空白组椎间盘组织结构清晰,髓核居中,纤维环排列规则,软骨终板结构完整。模型组髓核组织被纤维组织和成纤维细胞替代,纤维环较空白组粗糙增厚,排列不规则,出现裂隙,软骨终板不完整。针刀组髓核居中,与纤维环界限清晰,软骨终板结构正常。与空白组相比,模型组椎间盘组织p38MAPK蛋白与IL-1表达水平明显升高(P<0.01);与模型组相比,针刀组椎间盘组织p38MAPK蛋白表与IL-1达水平显著降低(P<0.01)。结论 针刀疗法可通过下调p38MAPK与IL-1的表达水平以延缓椎间盘退变。Objective To observe the effect of needle knife therapy on the expressions of p38 MAPK protein and IL-1 in degenerated cervical disc tissues of rabbits and explore the mechanism of needle knife treatment on cervical disc degeneration.Methods Twenty-four common grade 6-month-old healthy male New Zealand white rabbits were randomly divided into a blank group,a model group and a needle knife group,each containing 8 rabbits.The model group and needle knife group were used to make a cervical disc degeneration model using a home-made rabbit box.After 4 weeks of modeling,the success of modeling was evaluated by X-ray and MRI examination.Seven days after the end of modeling,the blank group and the model group received no intervention.The needle knife group received the needle knife intervention,1 time per week,totally 3 times.Seven days after the intervention,2 rabbits were randomly selected in each group for MRI to observe radiographic changes.At the end of the imaging examination,the intervertebral disc tissues were immediately removed after sacrifice by the auricular vein air embolism method.The histopathological changes were observed by HE staining,the protein expression level of p38 MAPK was detected by Western blot assay,and the expression level of IL-1 was detected by immunohistochemical staining.Results MRI showed that in the blank group,the height of intervertebral disc was normal,the nucleus pulposus was bright white,and no narrowing of intervertebral space and protrusion of intervertebral disc were found.The rim of annulus fibrosus was complete.In the model group,the intervertebral disc height was decreased,the nucleus pulposus was grayish black,the intervertebral space was narrowed,the C6/7 intervertebral disc protruded backward,and the annulus fibrosus was broken.In the needle knife group,the intervertebral disc height was normal,the nucleus pulposus was gray,the intervertebral space was not narrowed,the C6/7 intervertebral disc slightly protruded backward,and the rim of the annulus fibrosus was still intact

关 键 词：颈椎病 颈椎间盘退变 动物实验 兔 p38MAPK蛋白激酶 白细胞介素-1 

分 类 号：R245.31[医药卫生—针灸推拿学]