CNS Organoid Surpasses Cell-Laden Microgel Assembly to Promote Spinal Cord Injury Repair  

作　　者：Zitian Wang Haoran Zhao iaowei Tang Tianyu Meng Davit Khutsishvili Bing Xu Shaohua Ma 

机构地区：[1]Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China [2]Tsinghua-Berkeley Shenzhen Institute,Shenzhen 518055,China [3]Shenzhen Bay Laboratory,Shenzhen,China [4]Institute for Brain and Cognitive Sciences,Tsinghua University,Beijing 100084,China

出　　处：《Research》2022年第4期677-692,共16页研究（英文）

基　　金：This work was supported by the National Natural Science Foundationof China(grant numbers61971255 and 82111530212);the Natural Science Foundation of Guangdong Province(grant number 2021B1515020092);the Shenzhen Science and Technology Innovation Commission(grant numbersKCXFZ20200201101050887,RCYX20200714114736146,RCBS20200714114911104,and WDZC20200821141349001);the Shenzhen Bay Laboratory Fund(grant number SZBL2020090501014).

摘　　要：The choice of therapeutic agents remains an unsolved issue in the repair of spinal cord injury.In this work,various agents and configurations were investigated and compared for their performance in promoting nerve regeneration,including bead assembly and bulk gel of collagen and Matrigel,under acellular and cell-laden conditions,and cerebral organoid(CO)as the in vitro preorganized agent.First,in Matrigel-based agents and the CO transplantations,the recipient animal gained more axon regeneration and the higher Basso,Beattie,and Bresnahan(BBB)scoring than the grafted collagen gels.Second,new nerves more uniformly infiltrated into the transplants in bead form assembly than the molded chunks.Third,the materials loaded the neural progenitor cells(NPCs)or the CO implantation groups received more regenerated nerve fibers than their acellular counterparts,suggesting the necessity to transplant exogenous cells for large trauma(e.g.,a 5 mm long spinal cord transect).In addition,the activated microglial cells might benefit from neural regeneration after receiving CO transplantation in the recipient animals.The organoid augmentation may suggest that in vitro maturation of a microtissue complex is necessary before transplantation and proposes organoids as the premium therapeutic agents for nerve regeneration.

关 键 词：neural gained IMPLANTATION 

分 类 号：R651.2[医药卫生—外科学]