Genome-wide association study and metabolic pathway prediction of barrenness in maize as a response to high planting density  

作　　者：ZHANG Xu-huan LIU Hao MA Xu-hui ZHOU Gu-yi RUAN Hong-qiang CUI Hong-wei PANG Jun-ling KHAN Ullah Siffat ZONG Na WANG Ren-zhong LENG Peng-fei ZHAO Jun 

机构地区：[1]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China [2]State Key Laboratory of Vegetation and Environmental Change,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第12期3514-3523,共10页农业科学学报（英文版）

基　　金：the 2020 Research Program of Sanya Yazhou Bay Science and Technology City,China(SKJC-2020-02-005);the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202004 and CAAS-ZDRW202109).

摘　　要：Increasing the planting density is one way to enhance grain production in maize.However,high planting density brings about growth and developmental defects such as barrenness,which is the major factor limiting grain yield.In this study,the barrenness was characterized in an association panel comprising 280 inbred lines under normal(67500 plants ha–1,ND)and high(120000 plants ha–1,HD)planting densities in 2017 and 2018.The population was genotyped using 776254 single nucleotide polymorphism(SNP)markers with criteria of minor allele frequency>5%and<20%missing data.A genome-wide association study(GWAS)was conducted for barrenness under ND and HD,as well as the barrenness ratio(HD/ND),by applying a Mixed Linear Model that controls both population structure and relative kinship(Q+K).In total,20 SNPs located in nine genes were significantly(P<6.44×10–8)associated with barrenness under the different planting densities.Among them,seven SNPs for barrenness at ND and HD were located in two genes,four of which were common under both ND and HD.In addition,13 SNPs for the barrenness ratio were located in seven genes.A complementary pathway analysis indicated that the metabolic pathways of amino acids,such as glutamate and arginine,and the mitogen-activated protein kinase(MAPK)signaling pathway might play important roles in tolerance to high planting density.These results provide insights into the genetic basis of high planting density tolerance and will facilitate high yield maize breeding.

关 键 词：Zea mays L high planting density barrenness genome-wide association study MAPK pathway 

分 类 号：S513[农业科学—作物学]