外侧隔核背侧部生长抑素阳性神经元参与异氟醚麻醉觉醒  

Somatostatin positive neurons in the dorsal part of the lateral septal nucleus participate in isoflurane anesthesia-arousal

作　　者：王宇浩 张思敏 李健楠 董海龙 WANG Yuhao;ZHANG Simin;LI Jiannan;DONG Hailong(Central Laboratory,Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712046,China;Department of Anesthesiology and Perioperative Medicine,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]陕西中医药大学第二附属医院中心实验室,陕西咸阳712046 [2]空军军医大学西京医院麻醉与围术期医学科,陕西西安710032

出　　处：《空军军医大学学报》2022年第8期952-956,共5页Journal of Air Force Medical University

基　　金：国家自然科学基金(82030038)。

摘　　要：目的使用特异性顺向病毒示踪技术观察转基因小鼠外侧隔核背侧部(LSD)内生长抑素(SOM)阳性神经元的下游投射脑区,并使用神经元钙信号分析技术,观察其神经元活性在异氟醚麻醉觉醒中的变化。方法①特异性顺向示踪病毒AAV_(2/9)-Ef1α-DIO-mCherry被注射至SOM-Cre小鼠的LSD。病毒表达3周后将小鼠灌注取脑,并将全脑切成厚度为40μm的冠状脑片,进行全玻片免疫荧光成像扫描,观察LSD内SOM阳性神经元的下游投射脑区分布。②将特异性钙信号病毒AAV_(2/9)-Ef1α-DIO-GCaMP6s注射至SOM-Cre小鼠的LSD。病毒表达后,使用钙信号记录系统记录分析异氟醚麻醉觉醒过程中LSD内SOM阳性神经元的钙信号变化情况。结果LSD内SOM阳性神经元主要向外侧下丘脑、腹侧被盖区、前下丘脑核和前扣带回皮质发出密集投射。LSD内SOM阳性神经元的钙信号活动被异氟醚麻醉抑制,并且在意识消失过程中显著下降(P<0.05),在意识恢复过程中显著上升(P<0.05)。结论LSD内SOM阳性神经元主要向下丘脑、中脑和前皮质区发出投射。LSD内SOM阳性神经元参与异氟醚麻醉觉醒过程。Objective The downstream projection brain regions of somatostatin(SOM)positive neurons in the dorsal part of the lateral septal nucleus(LSD)were observed by neuronal-specific anterograde tracing in SOM-Cre mice.Neuronal calcium signaling analysis was used to observe the neuronal activity changes of SOM positive neurons in the LSD during isoflurane anesthesia-arousal.Methods①Anterograde tracing virus AAV_(2/9)-Ef1α-DIO-mCherry was injected into the LSD in SOM-Cre mice.Three weeks after virus expression,mice were perfused to take the brain,and the whole brain was cut into 40μm coronal slices.The whole-slide immunofluorescence imaging was performed to observe the distribution of SOM positive neurons in the downstream projection brain regions of LSD.②The specific calcium signaling virus AAV_(2/9)-DIO-GCaMP6s was injected into the LSD of SOM-Cre mice.After virus expression,calcium signaling recording system was used to record and analyze the changes of calcium signals of SOM positive neurons in the LSD during isoflurane anesthesia-arousal.Results SOM positive neurons in the LSD mainly projected to the lateral hypothalamus,ventral tegmental area,anterior hypothalamic nucleus and anterior cingulate cortex.The calcium signaling activity of SOM positive neurons in the LSD was inhibited by isoflurane anesthesia,and decreased significantly during loss of consciousness(P<0.05)and increased significantly during recovery of consciousness(P<0.05).Conclusion SOM positive neurons in the LSD mainly project to hypothalamus,midbrain and anterior cortex.SOM positive neurons in the LSD participate in isoflurane anesthesia-arousal process.

关 键 词：外侧隔核 生长抑素 异氟醚麻醉 觉醒 钙信号 

分 类 号：R614[医药卫生—麻醉学] R971.2[医药卫生—外科学]