人ARNTL基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of human ARNTL

作　　者：邱丽芬 陈睿博 张芯仪 海那尔·乌拉孜巴依 王芊芊 张茂祥 QIU Li-fen;CHEN Rui-bo;ZHANG Xin-yi;WULAZIBAYI Hainaer;WANG Qian-qian;ZHANG Mao-xiang(School of Basic Medical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;College of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,China;Clinical Medical College of Acupuncture-moxibustion and Rehabilitation,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学基础医学院,广东广州510006 [2]广州中医药大学中药学院,广东广州510006 [3]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046 [4]广州中医药大学针灸康复临床医学院,广东广州510006

出　　处：《生物技术》2022年第5期537-544,557,共9页Biotechnology

基　　金：国家自然科学基金面上项目(31771300);大学生创新创业训练计划项目(202110572022)。

摘　　要：[目的]克隆人ARNTL基因并分析其生物学特性。[方法]通过NCBI数据库中的人ARNTL基因设计引物,进行PCR扩增,将其连接在pGEX-4T-1载体上,然后通过双酶切及测序鉴定克隆的正确性,再使用在线软件分析预测人ARNTL的生物学特性。[结果]酶切结果显示人ARNTL基因开放阅读框为1878 bp,编码625个氨基酸残基,分子量为68690.67 Da,pI为6.40。ARNTL蛋白是主要位于细胞核中的亲水性蛋白,不含跨膜结构域,无信号肽,其二级结构由α螺旋、无规则卷曲和延伸链构成,并且三级结构与二级结构预测结果一致。[结论]成功克隆了人ARNTL基因,并对人ARNTL蛋白进行了生物信息学分析,为深入研究该蛋白的分子作用机理打下一定基础。[Objective]To clone the human ARNTL(Aryl hydrocarbon receptor nuclear translocator-like protein 1)gene and analyze its biological characteristics.[Method]The specific primers were designed based on the human ARNTL gene sequence from NCBI databases and target gene was amplified by PCR and ligated into the pGEX-4T-1 vector.The biological characters of human ARNTL were further analyzed by using bioinformatics tools.[Result]The enzyme digestion results showed that the 1878 bp length human ARNTL gene was cloned which encoding the 625 amino acid residues.The human ARNTL protein was predicting a stable hydrophilic protein localized in the nucleus,which does not contain a signal peptide and transmembrane domains,which secondary structure was consists ofα-helix,random curl and extended chain,and the predicting results of the tertiary structure and secondary structure were highly consistent.[Conclusion]Human ARNTL gene was successfully cloned and bioinformatics analysis was carried out to which lay a foundation for further study of the molecular mechanism of the protein.

关 键 词：人ARNTL基因 人ARNTL蛋白 克隆 生物信息学 预测和分析 

分 类 号：Q785[生物学—分子生物学]