中国北黄海地区海洋污损生物膜的微生物群落结构  

Microbial community structure in marine biofouling biofilms in Yellow Sea of China

作　　者：于慧瑛 王建华[2] 李新[1] YU Hui-ying;WANG Jian-hua;LI Xin(Life Sciences Department,Yuncheng University,Yuncheng 044000,China;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China)

机构地区：[1]运城学院生命科学系,山西运城044000 [2]中国科学院烟台海岸带研究所,山东烟台264003

出　　处：《生物技术》2022年第5期551-557,共7页Biotechnology

基　　金：国家自然科学基金项目(41907152);山西省高等学校科技创新项目(2019L0873);山西省服务产业创新学科群建设计划(特色农产品发展学科群)。

摘　　要：[目的]了解中国北黄海地区海污损生物膜的微生物群落组成。[方法]通过在烟台海域实地挂板,取得了附着1~7 d的污损生物膜,采用SEM显微分析技术确定了不同时间形成的污损生物膜的形貌特征,并利用16S rDNA高通量测序技术对污损生物膜的微生物群落结构进行了测序分析。[结果]高通量测序共得到214112条有效reads。不同附着时间形成的生物膜中微生物种类不同,但也有635种共有物种。不同时间形成的污损生物膜上有各自典型的附着菌属,如1 d的Tropicibacter、Neptuniibacter、Roseobacter,3 d的Aquibacter,5 d的Ruegeria,7 d的Actibacter、Robiginitalea、Sulfitobacter等。附着时间至7 d时,微生物群落组成逐渐趋于稳定,其中优势菌群为Chloroplast、Rhodobacteraceae和Marivita。[结论]不同附着时间形成的生物膜的表观形貌和群落组成有显著差异。挂板1 d后即有细菌附着,5 d后有硅藻附着。在1、3、5、7 d所形成的污损生物膜中发现了1346、1249、1540、1530个不同的物种。研究结果将对认识海洋污损生物膜的组成及功能,寻找有效的抗污手段等具有重要意义。[Objective]To study the microbial composition of the biofouling biofilms in marine environment of Yellow Sea of China.[Method]The biofouling biofilms formed for 1-7 days were collected from Yantai sea area.SEM microscopic analysis was used to determine the morphology characteristics of the biofouling biofilms formed at different times.The 16S rDNA high-throughput sequencing technology was used to analyze the microbial community structure of the biofouling biofilm.[Result]A total of 214,112 effective reads were obtained by high-throughput sequencing.There were 635 common species in biofilms with different adhesion time.It was found that the biofouling biofilms formed at different time had their own typical bacteria,such as Tropicibacter,Neptuniibacter,Roseobacter for 1 days,Aquibacter for 3 days,Ruegeria for 5 days,and Actibacter,Robiginitalea,Sulfitobacter for 7 days.The composition of microbial community gradually stabilized when the adhesion time reached 7 days,and the dominant bacteria were Chloroplast,Rhodobacteraceae and Marivita.[Conclusion]The morphology and the microbial community of the biofouling biofilms formed at different time vary markedly.Bacteria adhesion occurred after 1 day,and diatoms adhesion occurred after 5 days.There were 1346,1249,1540 and 1530 different species detected on the biofouling biofilms formed at 1st day,3rd day,5 th day and 7th day.

关 键 词：生物膜 生物污损 高通量测序 聚类分析 微生物群落结构 中国北黄海 

分 类 号：Q938[生物学—微生物学]