脑卒中后抑郁患者肠道菌群分布及其与血清IGF-1、BDNF、NGF、hcy水平的关系  

Distribution of intestinal flora in patients with post-stroke depression and its relationship with serum IGF-1,BDNF,NGF and hcy levels

作　　者：黄维翠 李柏新[2] 刘振宁[3] HUANG Wei-cui;LI Bo-xin;LIU Zhen-ning(School of Clinical Medicine,China Medical University,Shenyang,Liaoning 110000,China;First Department of Rehabilitation,Shengjing Hospital of China Medical University;First Emergency Department,Shengjing Hospital of China Medical University)

机构地区：[1]中国医科大学临床医学院,辽宁沈阳110000 [2]中国医科大学附属盛京医院第一康复科 [3]中国医科大学附属盛京医院第一急诊科

出　　处：《中国病原生物学杂志》2022年第11期1341-1344,共4页Journal of Pathogen Biology

摘　　要：目的检测脑卒中后抑郁(PSD)患者肠道菌群分布及其与血清胰岛素样生长因子-1(IGF-1)、脑源性神经营养因子(BDNF)、神经生长因子(NGF)、同型半胱氨酸(hcy)水平的关系。方法以2019年1月-2021年12月本院收治的213例脑卒中患者为对象,其中单纯脑卒中126例(对照组),PSD患者87例(研究组),日本光冈知足法检测粪便中乳酸杆菌、克雷伯菌、梭菌、Eggerthella菌、嗜血菌、反刍菌、毛螺菌、光冈菌、罗氏菌、肠杆菌、粪球菌、巴斯德氏菌含量;采用酶联免疫吸附(ELISA)法检测血清中IGF-1、BDNF、NGF水平;采用全自动生化分析仪检测血清中hcy水平;采用Pearson相关性分析法分析PSD患者肠道菌群含量与血清IGF-1、BDNF、NGF、hcy水平的相关性。结果与对照组相比,研究组患者粪便中的乳酸杆菌、克雷伯菌、肠杆菌含量及血清hcy水平均显著升高(均P<0.01),粪便中嗜血菌、反刍菌、毛螺菌、光冈菌、罗氏菌、巴斯德菌含量以及血清中IGF-1、BDNF、NGF水平均显著降低(均P<0.01)。研究组患者血清中IGF-1水平与嗜血菌、反刍菌、光冈菌、罗氏菌、巴斯德菌含量呈正相关,与肠杆菌含量呈负相关(均P<0.01);血清中BDNF水平与乳酸杆菌、克雷伯菌含量呈负相关,与反刍菌、光冈菌、罗氏菌、巴斯德氏菌含量呈正相关(均P<0.01);血清NGF水平与乳酸杆菌、克雷伯菌、肠杆菌含量呈负相关,与光冈菌、巴斯德菌含量呈正相关(均P<0.01);血清hcy水平与乳酸杆菌、克雷伯菌、肠杆菌含量呈正相关,与嗜血菌、反刍菌、光冈菌、罗氏菌、巴斯德菌含量呈负相关(均P<0.01)。结论PSD患者肠道菌群分布与血清IGF-1、BDNF、NGF、hcy水平密切相关,检测血清IGF-1、BDNF、NGF、hcy水平能反映肠道菌群分布,可为疾病的早期防治提供参考。Objective To detect the distribution of intestinal flora in patients with post-stroke depression(PSD)and its relationship with serum insulin-like growth factor-1(IGF-1),brain-derived neurotrophic factor(BDNF),nerve growth factor(NGF),and homocysteine(hcy)levels.Methods From January 2019 to December 2021,213 stroke patients in our hospital were collected as the research objects,including 126 stroke patients(control group)and 87 PSD patients(study group).The contents of Lactobacillus,Klebsiella,clostridium,Eggerthella,Haemophilus,Ruminobacter,Lachnospira,Mitsuokella,Rothia,Enterobacter,Coprococcus,Pasteurella in feces were measured using Japan Mitsuoka contentment method;the levels of IGF-1,BDNF and NGF in serum were detected by enzyme-linked immunosorbent assay(ELISA);the level of hcy in serum was detected by an automatic biochemical analyzer;the correlation between intestinal flora contents and serum levels of IGF-1,BDNF,NGF and hcy in PSD patients was analyzed by Pearson correlation analysis.Results Compared with the control group,the contents of Lactobacillus(3.89±0.91 vs.2.39±0.56),Klebsiella(9.36±1.15 vs.8.48±0.76)and Enterobacter(2.26±0.35 vs.1.22±0.26),and the level of hcy(23.48±5.36 vs.18.25±3.47)mmol/L in serum increased in the study group(t=14.875,6.725,24.873,8.643,all P<0.01),the contents of Haemophilus(12.45±3.19 vs.16.37±4.06),Ruminobacter(0.35±0.16 vs.0.63±0.17),Lachnospira(0.48±0.09 vs.1.39±0.71),Mitsuokella(0.36±0.11 vs.0.57±0.26),Rothia(0.45±0.33 vs.0.86±0.21),and Pasteurella(3.16±0.42 vs.4.95±0.87),and the levels of serum IGF-1(30.65±6.74 vs.43.47±8.59)ng/mL,BDNF(7.84±0.93 vs.13.56±3.48)ng/mL and NGF(286.88±53.17 vs.373.51±83.89)pg/mL decreased(t=7.529,12.101,11.881,7.103,11.077,17.803,11.659,14.957,8.519,all P<0.01).The level of IGF-1 in serum of patients with PSD was positively correlated with the contents of Haemophilus,Ruminobacter,Mitsuokella,Rothia and Pasteurella,and negatively correlated with the content of Enterobacter(all P<0.01);the level of BDNF in serum was

关 键 词：脑卒中后抑郁 肠道菌群 胰岛素样生长因子-1 脑源性神经营养因子 神经生长因子 同型半胱氨酸 

分 类 号：R378[医药卫生—病原生物学]