Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats  

作　　者：Dina Tucovic Jelena Kulas Ivana Mirkov Dusanka Popovic Lidija Zolotarevski Marta Despotovic Milena Kataranovski Aleksandra Popov Aleksandrov 

机构地区：[1]Immunotoxicology Group,Department of Ecology,Institute for Biological Research"Sinisa Stankovic"-National Institute of Republic of Serbia,University of Belgrade,142 Bulevar despota Stefana,11060 Belgrade,Serbia [2]Medical College of Applied Sciences,254 Cara Dusana,11080 Belgrade,Serbia [3]Centre of Research Excellence in Nutrition and Metabolism,Institute for Medical Research,University of Belgrade,11 Tadeusa Koscuska 1,11000 Belgrade,Serbia

出　　处：《Biomedical and Environmental Sciences》2022年第11期1038-1050,共13页生物医学与环境科学（英文版）

基　　金：supported by the Ministry of Education,Science and Technological Development of the Republic of Serbia[451-03-68/2020-14/200007]。

摘　　要：Objective The effect of oral cadmium(Cd)intake to influence contact skin allergies was examined,since it is known that Cd is a heavy metal that affects many tissues,including the skin,in which it disturbs homeostasis,thus resulting in inflammation and injury.Methods Male rats were evoked with experimental contact hypersensitivity reaction(CHS)to hapten dinitrochlorobenzene(DNCB),after prolonged(30 day)oral exposure to an environmentally relevant Cd dose(5 ppm).The ear cell population was analyzed with flow cytometry.Cytokine production by ear skin cells and the activity of skin-draining lymph node(DLN)cells were measured using enzyme-linked immunosorbent assay(ELISA).Results Orally acquired Cd(5 ppm)increased CHS intensity only in Dark Agouti(DA)rats by affecting inflammatory responses in both the sensitization(an increase of IFN-γ and IL-17 cytokine production)and challenge(an increase of CD8^(+)and CD4^(+)cell number and TNF,IFN-γ and IL-17 cytokine production)phases.An increased CHS reaction was seen in Albino Oxford(AO)rats only at a high Cd dose(50 ppm),during the challenge phase(an increase of CD8^(+)and CD4^(+)cell number and TNF,IFN-γ and IL-17 cytokine production).Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals such as DNCB.

关 键 词：Cadmium(Cd) Contact hypersensitivity reaction(CHS) Dinitrochlorobenzene(DNCB) 

分 类 号：R99[医药卫生—毒理学]