基于转录组的芥蓝NAC家族成员鉴定与分析  

Identification and Analysis of NAC Factor Family based on the Transcriptome of Chinese kale(Brassica oleracea)

作　　者：刘涛[1] 陈家璇 Gefu Wang-Pruski 郭容芳[1,2] Liu Tao;Chen Jiaxuan;Gefu Wang-Pruski;Guo Rongfang(Joint FAFU-Dalhousie Lab,College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,350002;Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,350002;Faculty of Agriculture,Dalhousie University,Truro,NS B2N 5E3,Canada)

机构地区：[1]福建农林大学园艺学院,戴尔豪西大学联合实验室,福州350002 [2]福建农林大学园艺学院,园艺植物生物工程研究所,福州350002 [3]戴尔豪西大学农学院,特鲁罗,NSB2N5E3,加拿大

出　　处：《分子植物育种》2022年第22期7332-7341,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31401859,31772310);博士后启动经费(132300155);博士后第10批特别项目(2017-T100464)共同资助。

摘　　要：本研究运用生物信息学方法,基于转录组数据在甘蓝(Brassica oleracea)基因组中鉴定了28个芥蓝(Brassica alboglabra)NAC家族基因,对该家族成员蛋白基本理化信息、内含子、蛋白保守结构域、MOTIF基序、系统进化树、启动子顺式作用元件和转录组表达量等进行分析。理化性质分析表明,28个BoNAC蛋白序列长度为139~556 aa,16.64~62.27 kD,等电点(pI)在4.5~10.06;拟南芥(Arabidopsis thaliana)和芥蓝的NAC转录因子家族系统发育树分析显示芥蓝和拟南芥NAC基因可分为17个亚族;MEME和保守结构域分析表明28个BoNAC基因都具有NAM结构域的保守基序结构;启动子分析表明BoNAC家族成员受到多种植物激素、胁迫和光的调控;BoNAC家族28个基因在高温处理下转录组中的表达量表明高温上调了大部分BoNAC家族基因的表达量。研究结果对BoNAC家族的后续研究具有一定的意义。In this study,28 Chinese kale(Brassica alboglabra)NAC family genes were identified using bioinformatics methods in the genome of Brassica oleracea based on transcriptome data,and the basic physical and chemical information,introns,protein conserved structure of the family members’proteins domains,motifs,phylogenetic trees,promoter cis-acting elements and transcriptome expression levels were analyzed.The analysis of physical and chemical properties showed that 28 BoNAC protein sequences were 139~556 aa,length were 16.64~62.27 kD,and isoelectric point(p I)were between 4.5 and 10.06.Arabidopsis thaliana and Chinese kale NAC gene family phylogenetic tree analysis showed that the NAC genes of Chinese kale and Arabidopsis can be divided into 17 subfamilies.MEME and conserved domain analysis showed that 28 BoNAC genes all have the conserved motif structure of NAM domain.Cis-acting elements analysis showed BoNAC were regulated by plant hormones,stress and light.The expression of 28 BoNAC family genes in the transcriptome under high temperature treatment indicated that high temperature up-regulates the expression of m ost BoNAC family genes.This research results have cer tain sig-nificance for the follow-up research of the BoNAC family.

关 键 词：芥蓝 NAC 生物信息学 表达分析 

分 类 号：S635.9[农业科学—蔬菜学]