文心兰花瓣转录组分析与调控花瓣衰老关键基因挖掘  

Transcriptome Analysis of Oncidium Petals and Mining of Key Genes Regulating Petal Senescence

作　　者：李晨 裴小华 王帅[1] 曲飞鸿 薛唯佳 徐丹 李梦桃 王鹏[1] 张恒[1] 潘英文[2] 夏志强 刘进平[1] Li Chen;Pei Xiaohua;Wang Shuai;Qu Feihong;Xue Weijia;Xu Dan;Li Mengtao;Wang Peng;Zhang Heng;Pan Yingwen;Xia Zhiqiang;Liu Jinping(Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources,College ofTropical Crops,Hainan University,Haikou,570228;Post-Entry Quarantine Station for Tropical Plant,Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou,570311)

机构地区：[1]海南大学热带作物学院,海南省热带生物资源可持续利用重点实验室,海口570228 [2]海南出入境检验检疫局,热带植物隔离检疫中心,海口570311

出　　处：《分子植物育种》2022年第22期7354-7364,共11页Molecular Plant Breeding

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC147);海南省重点研发计划项目(ZDYF2020053);五指山市科技和工业信息化局项目(WZSKTPXM2021005);国家自然科学基金项目(31560573)共同资助。

摘　　要：花瓣衰老是观赏园艺植物具有经济价值的重要性状。为了解文心兰衰老花瓣的转录组学特征,挖掘参与调控文心兰花瓣衰老的关键基因,本研究对盛开期(E)、衰老初期(F)和衰老期(G)的文心兰切花花瓣进行RNA测序。测序序列经组装拼接后,共获得48661个Unigenes,在NR、NT、SwissPort、KEGG、COG、InterPro、GO数据库中分别注释到34922(71.77%)、26286(54.02%)、23134(47.54%)、25678(52.77%)、14483(29.76%)、25580(52.57%)和9103(18.70%)条Unigenes。经表达量比较,在盛开期(E)和衰老初期(F)之间获得差异表达基因3914条,衰老初期(F)和衰老期(G)之间获得差异表达基因1579条。经GO功能富集分析,花瓣盛开期(E)和衰老初期(F)共有3914、衰老初期(F)和衰老期(G)之间共有1579条差异表达基因被注释参与生物学过程、细胞组分和分子功能三个功能组。KEGG功能富集分析表明,盛开期(E)和衰老初期(F)之间的差异表达基因参与18个代谢通路,而衰老初期(F)和衰老期(G)之间的差异表达基因参与7个代谢通路。鉴定出可能在文心兰花花瓣衰老发挥关键作用的一批包括乙烯、生长素、赤霉素、茉莉酸、脱落酸及细胞分裂素在内的植物生长调节物质的合成与信号转导相关基因和转录因子基因。本研究所获得的文心兰花瓣衰老转录组数据,为进一步鉴定衰老相关基因的功能,了解花瓣衰老分子机制打下基础。Petal senescence is an important trait of economic value in ornamental plants.In order to understand the transcriptomic characteristics of the senescent petals and explore the key genes involved in regulating petal senescence,RNA sequencing of the petals of Oncidium cut flowers at the stages of full opening(E),initial senescence(F)and senescence(G)was conducted.Of 48661 Unigenes detected,34922(71.77%),26286(50.02%),23134(47.54%),25678(52.77%),14483(29.76%),25580(52.57%)and 9103(18.70%)Unigenes were annotated in NR,NT,SwissPort,KEGG,COG,InterPro and GO databases respectively.After comparing the expression levels of the Unigenes,3914 differentially expressed genes(DGEs)were obtained between the full opening samples and the initial senescence samples(F/E),and 1579 DGEs were obtained between the initial senescence samples and the senescence samples(G/F).GO functional enrichment analysis showed that 3914 DGEs of F/E and 1579 DGEs of G/F were annotated to three functional groups:biological process,cellular component and molecular function.KEGG function enrichment analysis showed that the DGEs of F/E were involved in 18 metabolic pathways,while the DGEs of G/F were involved in 7 metabolic pathways.A number of transcription factor(TF)genes and the genes related to the biosynthesis and signal transduction of plant growth regulators including ethylene(ETH),auxin(IAA),gibberellin(GA),jasmonic acid(JA),abscisic acid(ABA)and cytokinin(CDK)were identified for their potential modulatory functions in the petal senescence of Oncidium.The transcriptome data obtained in this study lays a foundation for further functional identification of senescence-related genes and elucidation of the molecular mechanism of petal senescence.

关 键 词：文心兰(Oncidium) 花瓣衰老 RNA测序 转录组 

分 类 号：S682.31[农业科学—观赏园艺]