水稻抗褐飞虱基因Bph9共显性功能标记开发及应用  

Development and Validation of a Functional Codominant Marker for the Bph9 Gene in Rice

作　　者：陈明亮 熊文涛 罗世友 沈雨民 熊焕金 吴小燕 肖叶青 Chen Mingliang;Xiong Wentao;Luo Shiyou;Shen Yumin;Xiong Huanjin;Wu Xiaoyan;Xiao Yeqing(National Rice Engineering Laboratory of China,Nanchang Branch,National Rice Improvement Center of China,Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang,330200)

机构地区：[1]江西农业科学院水稻研究所,国家水稻改良工程中心南昌分中心,水稻国家工程实验室,南昌330200

出　　处：《分子植物育种》2022年第22期7482-7487,共6页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2016YFD0101801);江西省重大科技研发专项(20203ABC28W013)共同资助。

摘　　要：褐飞虱(Nilaparvata lugens St?l,BPH)是亚洲最具有毁灭性的水稻虫害。培育具有褐飞虱抗性的水稻品种是最有效经济防控褐飞虱的方法。Bph9定位在第12号染色体,表现出广谱的褐飞虱抗性,具有较好的应用前景。但在Bph9遗传位点,存在有多个褐飞虱抗性较弱的等位基因,前期缺少有效的标记难以鉴定。本研究通过比较Bph9和其他等位基因,发现在Bph9阅读框中存在一个15个碱基的缺失。基于这个缺失,设计开发了一个共显性功能标记Bph9-M,可以区别Bph9和其他感虫和抗虫等位基因,高效选择带有Bph9的抗性植株。该标记有助于利用Bph9辅助育种培育抗褐飞虱品种。Brown planthopper(Nilaparvata lugens St?l,BPH)is the most destructive rice insect pests in Asia.Breeding BPH-resistant varieties is the most effective and economical way to control BPH.Bph9,located on chromosome 12,was considered to have diverse application prospects due to its broad-spectrum resistance to the BPH.However,in its genetic locus,there are multiple alleles with reduced BPH resistance.In this study,a 15 bp deletion was found in the open reading frame of Bph9 by compareing Bph9 to other alleles.Based on this deletion,we developed a codominant functional indel marker,Bph9-M,that can distinguish Bph9 from susceptible alleles and other resistant alleles,to select the BPH-resistant rice lines with Bph9 efficiently.This marker is a helpful tool for breeding BPH-resistant rice via marker-assisted selection of Bph9.

关 键 词：水稻 抗褐飞虱 Bph9 标记辅助选择 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]