氟吡菌胺及其代谢物在荔枝中的残留状况及安全使用评价  

Residue status and application safety assessment of fluopicolide and 2,6-dichlorobenzamide in litchi

作　　者：吴琼[1] 黄海珠[1] 陈博钰 韩丙军[1] 陈显柳 徐志[1] WU Qiong;HUANG Hai-zhu;CHEN Bo-yu;HAN Bing-jun;CHEN Xian-iu;XU Zhi(Analysis and Test Center,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Quality and SafetyControl for Subtropical Fruit and Vegetable(Haikou),Ministry of Agriculture and Rural Affairs,Key Laboratory of Tropical Fruits and Vegetables Qualityand SafetyforStateMarketRegulation,Haikou571101,China)

机构地区：[1]中国热带农业科学院分析测试中心,农业农村部亚热带果品蔬菜质量安全控制重点实验室(海口),国家市场监管重点实验室(热带果蔬质量与安全),海口571101

出　　处：《农药》2022年第12期900-904,共5页Agrochemicals

基　　金：海南省自然科学基金资助(322RC793);国家市场监管重点实验室(热带果蔬质量与安全)(ZX-2022002)。

摘　　要：[目的]为了解氟吡菌胺在荔枝中的残留情况和消解动力学规律,建立了荔枝中氟吡菌胺及其代谢物2,6-二氯苯甲酰胺(BAM)的残留分析方法。[方法]采用优化的QuEChERS方法,结合超高效液相色谱-串联质谱(UPLCMS/MS)建立了荔枝中氟吡菌胺及其代谢物BAM的检测方法,研究氟吡菌胺在荔枝中的残留消解动态及最终残留量。[结果]在添加水平0.01~1 mg/kg范围内,氟吡菌胺和BAM在荔枝全果和果肉中的平均回收率为86%~111%,相对标准偏差在2.1%~8.4%范围,定量限(LOQ)为0.01 mg/kg。氟吡菌胺在荔枝中的残留消解动态试验,海口和南宁2地消解半衰期分别为8.0、8.5 d。施药后14、21 d的荔枝全果和果肉样品中氟吡菌胺的残留量小于0.1 mg/kg,BAM均未检出。[结论]综合残留消解动态和最终残留试验,推荐20%氟吡菌胺悬浮剂用于防治荔枝霜霉病,于病害发生初期进行叶面喷雾处理,施药量为111 mg a.i./kg(制剂用量550 mg/kg),施药3次,施药间隔10 d,施药后14 d采收荔枝果实最为安全。[Aims]This study aims to understand the residues and dissipation kinetics of fluopicolide in litchi,a residue analysis method for fluopicolide and its metabolite 2,6-dichlorobenzamide(BAM)in litchi was established.[Methods]The optimized QuEChERS method combined with ultra performance liquid chromatoid-tandem mass spectrometry(UPLC-MS/MS)was used to establish a method for the detection of fluopicolide and its metabolite BAM in litchi,and the residual dissipation dynamics and final residues of fluopicolide in litchi were studied.[Results]In the range of 0.01-1 mg/kg,the average recoveries of fluopicolide and BAM in whole fruit and pulp of litchi were 86%-111%,the relative standard deviations were 2.1%-8.4%,and the LOQ was 0.01 mg/kg.In the dynamic experiment of residual dissipation of fluopicolide in litchi,the half-lives of the two sites in Haikou,Hainan and Nanning,Guangxi were 8.0and 8.5 d respectively.On day 14 and 21 after application,the residual amount of fluopicolide in whole fruit and pulp samples of litchi was less than 0.1 mg/kg,BAM was not detected.[Conclusions]Based on the results of residue dissipation and final residue test,fluopicolide 20%suspension is recommended for the control of litchi downy mildew.Foliar spray is applied at the initial stage of the disease,and the dosage is 111 mg a.i./kg(550 mg formulation/kg).The application interval is 10 d.It is safest to harvest litchis 14 d after application.

关 键 词：氟吡菌胺 2 6-二氯苯甲酰胺 荔枝 残留消解动态 最终残留 

分 类 号：TQ450.2[化学工程—农药化工]