马勃灌肠液对溃疡性结肠炎模型小鼠TNF-α/IL-6细胞因子的影响及疗效研究  

Effect of Mabo Enema on Cytokines of TNF-αand IL-6 in UC Model Mice

作　　者：杨爱龙[1] 阚成国[1] 韩雪[1] 喻少雷 孙凤娟 YANG Ailong;KAN Chengguo;HAN Xue;YU Shaolei;SUN Fengjuan(The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150001,China)

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001

出　　处：《中医药学报》2022年第12期32-37,共6页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省自然科学基金项目(H2017069);黑龙江中医药大学科研基金项目(201729)。

摘　　要：目的:研究马勃灌肠液对溃疡性结肠炎模型小鼠的作用及对TNF-α、IL-6细胞因子的影响,探讨其对溃疡性结肠炎的作用机制。方法:将48只小鼠随机分成两组,其中正常对照组6只、造模组42只。正常对照组给予普通饮用水6 d,模型组饮用含2.5%葡聚糖硫酸钠(DSS)的饮用水6 d,各组小鼠造模期间正常饮食。6 d后处死部分小鼠,评估造模情况,确定造模成功后将剩余模型组小鼠随机分成3组:模型组11只,马勃组12只,柳氮磺吡啶组12只,加上正常对照组共分为4组,给予相应溶液灌肠,连续5 d,共计10次。5 d后对小鼠进行DAI量表评估,随后处死小鼠,取材进行HE染色,观察病理情况,并采用ELISA法检测TNF-α、IL-6细胞因子浓度。结果:造模组较正常对照组DAI评分有明显增加(P<0.05);马勃组与柳氮磺吡啶组DAI评分均较模型组显著降低(P<0.05);马勃组与柳氮磺吡啶组差异无统计学意义(P>0.05)。正常对照组第6天与11天TNF-α与IL-6浓度基本无变化,模型组第6天与11天TNFα与IL-6浓度显著升高(P<0.05);柳氮磺嘧啶组、马勃组与模型组TNF-α、IL-6浓度相比,差异有统计学意义(P<0.05);分别对比观察马勃组、柳氮磺嘧啶组与模型组第11天TNFα与IL-6浓度,差异均具有统计学意义(P<0.05)。马勃组与柳氮磺嘧啶组TNFα、IL-6浓度相比,差异无统计学意义(P>0.05)。结果:马勃灌肠液可以显著降低溃疡性结肠炎小鼠TNF-α、IL-6细胞因子浓度,抑制炎症反应,对于溃疡性结肠炎可以起到明显的治疗作用,且马勃灌肠液与柳氮磺吡啶疗效相当。Objective:To study the effect of Mabo Enema on cytokines of TNF-αand IL-6 in mice with ulcerative colitis(UC),thus to investigate its action mechanism in treating UC.Methods:48 mice were randomly divided into the normal control group(n=6)and the modeling group(n=42).The normal group was given normal drinking water for 6 days,and the modeling group was given drinking water containing 2.5%dextran sodium sulfate(DSS)for 6 days,and the mice in each group were fed with normal diet during the modeling period.After 5 days,the successful modeling mice were randomly divided into the model group(n=11),the Mabo group(n=12)and the Salazosulfapyridine group(n=12).Each group was intervened with corresponding enema for 5 days.DAI scale was evaluated before the execution of the mice.HE staining was performed to observe the pathological changes,and ELISA method was used to measure the concentrations of TNF-αand IL-6.Results:DAI score was significantly increased in the modeling group compared with that in the normal control group(P<0.05);DAI score was significantly decreased in the Mabo group and the Salazosulfapyridine group compared with that in the model group after the treatment(P<0.05);there was no statistical difference in DAI between the Mabo group and the Salazosulfapyridine group(P>0.05).The concentrations of TNF-αand IL-6 were basically unchanged in the normal control group between day 6 and day 11,and the concentrations of TNF-αand IL-6 were significantly higher in the model group on day 6 and day 11(P<0.05);there were statistical differences in the concentrations of TNF-αand IL-6 between the Mabo group and the model group,as well as between the Salazosulfapyridine group and the model group on day 11(P<0.05);there were no statistical difference in the concentrations of TNF-αand IL-6 between the Mabo group and the Salazosulfapyridine group(P>0.05).Results:Mabo enema can significantly reduce the concentrations of TNF-αand IL-6 in mice with UC and inhibit the inflammatory response,which can play a significant therap

关 键 词：溃疡性结肠炎 马勃灌肠液 细胞因子 炎症介质 

分 类 号：R285.5[医药卫生—中药学]