2000-2021年江西省O1群和O139群霍乱弧菌非产毒株基因组学研究  

Genomic characteristics of non-toxigenic Vibrio cholerae O1 and O139 isolated in Jiangxi,2000-2021

作　　者：王鹏[1] 赵文轩 徐晓倩[1] 周海健[3] 熊长辉[1] 李小英 章承锋[1] 袁辉[1] 杨梦[1] Wang Peng;Zhao Wenxuan;Xu Xiaoqian;Zhou Haijian;Xiong Changhui;Li Xiaoying;Zhang Chengfeng;Yuan Hui;Yang Meng(Jiangxi Province Center for Disease Control and Prevention,Nanchang 330029,Jiangxi,China;School of Public Health,Shanxi Medical University,Taiyuan 030001,Shanxi,China;State Key Laboratory for Communicable Disease Prevention and Control,National Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]江西省疾病预防控制中心,江西南昌330029 [2]山西医科大学公共卫生学院,山西太原030001 [3]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《疾病监测》2022年第11期1419-1422,共4页Disease Surveillance

基　　金：江西省卫生计生委科技计划(No.202211302)。

摘　　要：目的分析2000—2021年江西省O1群和O139群霍乱弧菌非产毒株的基因组变异特征。方法使用基因组重测序技术获得菌株的基因组精细图,使用IQ-tree构建基于核心基因组单核苷酸多态性(SNP)的遗传系统发生树,使用脉冲场凝胶电泳(PFGE)研究密切相关菌株的差异,使用最小抑制浓度(MIC)测定菌株的抗生素敏感性。结果江西省的O1群和O139群霍乱弧菌非产毒株基因组多态性较引起霍乱流行和大流行的产毒株高,其中部分O1群霍乱弧菌非产毒株携带耐药质粒,表现出对多种抗生素的敏感性降低。结论O1群和O139群霍乱弧菌的基因组多态性高,应该密切关注携带多耐药质粒的霍乱弧菌非产毒株。Objective To investigate the characteristics of non-toxigenic Vibrio cholerae O1 and O139 isolated in Jiangxi province between 2000 and 2021.Methods The genome sequences of these strains were obtained with next generation sequencing;the cladogram was based on the SNPs in the core genome of these strains.Pulse field gel electrophoresis was used to investigate the variation of these strains.Minimum inhibitory concentration test was used to evaluate the susceptibility of these strains to antibiotics.Results The non-toxigenic V.cholerae O1 and O139 isolates in Jiangxi showed mire genome diversity compared with the toxigenic strains which caused cholera epidemic and pandemic.Some O1 non-toxigenic strains carried drug resistance plasmid,showing low sensitivity to multi antibiotics.Conclusion The genome diversity of nontoxigenic V.cholerae O1 and O139 was high.Surveillance for non-toxigenic V.cholerae strains carrying drug resistance plasmid should be carried out.

关 键 词：基因组 抗生素敏感性 质粒 

分 类 号：R211[医药卫生—中医学] R183[生物学—微生物学] R378.3Q93-31