一种肺炎链球菌转运培养基保存效果评估  

Efficacy of STGG-transfer medium for Streptococcus pneumoniae:an experimental evaluation

作　　者：周珊珊 王建 白霜[1,2] 吕敏 兰文文[1,2] 吴疆 ZHOU Shan-shan;WANG Jian;BAI Shuang(Institute for Immunizations and Vaccines,Beijing Center for Disease Prevention and Control,Beijing 100013,China)

机构地区：[1]北京市疾病预防控制中心免疫预防所,北京100013 [2]北京市预防医学研究中心

出　　处：《中国公共卫生》2022年第10期1291-1293,共3页Chinese Journal of Public Health

基　　金：北京市属医学科研院所公益发展改革试点项目(京医研2018-1)。

摘　　要：目的评估一种转运培养基,脱脂牛奶胰蛋白胨葡萄糖甘油(STGG)对于肺炎链球菌的保存效果。方法分别使用STGG和Todd-Hewitt酵母肉汤冻存液(THY,含20%甘油)将肺炎链球菌稀释相同倍数置于4℃保存48 h、1周,置于-80℃保存1、2个月,涂板培养比较菌落数差异;同时使用STGG培养基制备不同浓度肺炎链球菌菌液分别于-20℃和-80℃保存不同时间后涂板培养观察菌落数变化。反复冻融STGG培养基制备的肺炎链球菌菌液后涂板培养观察菌落数变化。结果肺炎链球菌在THY冻存液和STGG培养基中4℃保存48 h,菌落数量相比于0 h仅变化了1.9%;保存1周后,THY冻存液中肺炎链球菌菌落数量相比于48 h下降了70.9%,STGG培养基中下降了48.3%。不同浓度的肺炎链球菌在STGG培养基中-20℃及-80℃保存1个月菌落存活率差异无统计学意义(P>0.05),-80℃保存2个月存活率明显高于-20℃(P<0.05)。STGG培养基保存液-80℃冻融5次,与第一次冻融相比,菌落数变化在95%~102%以内。结论相较于THY冻存液,STGG培养基保存效果更好,且-80℃更利于肺炎链球菌于STGG培养基中的保存。Objective To evaluate the efficacy of skim milk-tryptone-glucose-glycerin(STGG)–a transport medium for Streptococcus pneumonia(Spn).Methods Bacterial suspensions of Spn at three dilution levels were prepared with STGG and Todd-Hewitt yeast broth containing 20%glycerol(THY)cryopreservation solution separately.All the suspensions were stored at 4°C for 48 hours and one week,at–80°C for one month and two months.Plate culture was adopted to assess the difference in colony numbers of preserved Spn with the two medium after repeated freezing and thawing;the variation in colony numbers was also observed for the preserved Spn with STGG stored at–20°C and–80°C for different period.Results The number of Spn colonies changed only by 1.9%after storage with THY cryopreservation medium and STGG at4°C for 48 hours compared to that before the storage;while after the storage of one week,the Spn colony number dropped by70.9%for the preservation with THY cryopreservation medium and 48.3%for with STGG.No significant difference was observed in survival rate of Spn preserved with STGG medium at different dilution levels stored at–20°C and–80°C for one month(P>0.05),but the survival rate was significantly higher for the Spn stored at–80°C than that at–20°C for2 months(P<0.05).For the Spn preservation stored at–80°C,STGG freezing and thawing 5 times resulted in a change from 95%–102%in the number of colonies.Conclusion Compared with THY cryopreservation solution,STGG medium results in a better efficacy for Streptococcus pneumoniae preservation,especially when stored at a temperature of–80°C.

关 键 词：培养基 肺炎链球菌 温度 

分 类 号：R183.3[医药卫生—流行病学]