基于适配体的杂交链式反应在检测中应用  

Application of aptamer-based hybridization chain reaction in detections

作　　者：苏柳 邓省亮[1] 贺伟华[1] 章钢刚 SU Liu;DENG Sheng-liang;HE Wei-hua(Department of Applied Microbiology,Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang,Jiangxi Province 330096,China)

机构地区：[1]江西省科学院微生物研究所,南昌330096

出　　处：《中国公共卫生》2022年第10期1364-1368,共5页Chinese Journal of Public Health

基　　金：国家自然科学基金(31960502);江西省重点研发计划项目(20202BBGL73119;20202BBFL63039);江西省科学院科技计划项目(2020-YZD-03)。

摘　　要：开发简单、灵敏的检测方法对医学诊断、环境监测、食品安全等领域的研究具有重要意义。适配体作为新型生物识别分子,与杂交链式反应(HCR)结合能实现高灵敏检测靶标物,形成一种新型检测模式。HCR是一种无需酶参与,常温下可自组装的扩增技术。基于适配体的HCR技术因具有高特异性、高灵敏性、操作简单、成本低廉等优点受到研究者们的广泛关注。本文主要介绍了HCR反应的基本原理及特征,重点综述了近年来基于适配体的HCR技术在蛋白质、酶活性、小分子及肿瘤细胞检测中的应用,探讨了该技术目前存在的主要问题,以期为建立高效率、高灵敏的适配体HCR检测系统提供理论参考。Developing simple and sensitive detection methods is of great significance to researches on medical diagnosis,environmental analysis and food safety.As a new biological indicator molecule,aptamers are combined with hybridization chain reaction(HCR)to form a new detection model for sensitive detection of analytes.HCR is a typical amplification technology,which can be carried out without enzyme and self-assembled at room temperature.The aptamer-based HCR technology has be attracted great interest due to its high specificity and sensitivity,simple protocol and low cost.The study reviews basic features of HCR with an emphasis on the application of aptamer-based HCR in detection,such as proteins,enzyme activities,small molecules and tumor cells.Major existing problems of the technology are also discussed.The review is aimed to provide a theoretical reference for establishing a highly efficient and sensitive HCR detection system.

关 键 词：适配体 杂交链式反应 高特异性 高灵敏性 检测 

分 类 号：R115[医药卫生—公共卫生与预防医学]