中医辟谷养生术对肠道菌群的调节作用研究  

Alterations of Gut Microbiome in Healthy Chinese Population by Chinese Medical Bigu

作　　者：郭建红 燕晓雯[3] 王俊磊[4] 马英锋[2] 杨延辉[3] 李保有[1,5] GUO Jian-hong;YAN Xiao-wen;WANG Jun-lei;MA Ying-feng;YANG Yan-hui;LI Bao-you(College of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan 750004;Key Laboratory of Ningxia Ethnomedicine modernization,Ministry of Education,Ningxia Medical University,Yinchuan 750004;Basic Medical College of Ningxia Medical University,Yinchuan 750004;Taishan Sanatorium of Shandong Province,Shandong 271000;Li baoyou’s Klinik,Maribo 4930),Danmark)

机构地区：[1]宁夏医科大学中医学院,银川750004 [2]宁夏医科大学少数民族医药现代化教育部重点实验室,银川750004 [3]宁夏医科大学基础医学院,银川750004 [4]山东省泰山疗养院,山东271000 [5]李保有中医中心,丹麦马里博4930

出　　处：《中国中西医结合杂志》2022年第11期1329-1334,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：宁夏医科大学校级重点研究项目(No.XZ2020005);宁夏科技厅宁夏自然科学基金项目(No.2021A0306);宁夏科技厅宁夏自然科学基金项目(No.2019AAC03091)。

摘　　要：目的观察中医辟谷养生术对肠道菌群的调节作用。方法共计23名受试者进行5~7天辟谷,同时练习养生功法。分别于辟谷前3天、辟谷后3天及2个月采集受试者粪便样本,对样本进行DNA抽提和检测、16S r DNA序列扩增、Mi Seq测序和数据分析。结果23名受试者中18名完成了辟谷干预及3次粪便样本采集。与辟谷前比较,辟谷后2个月拟杆菌丰度在门、纲、目水平均明显增多(P<0.05),厚壁菌门的毛螺菌科丰度明显减少(P<0.05),厚壁菌门/拟杆菌门比例由辟谷前的0.65±0.67,降至辟谷后0.43±0.25,以及辟谷后2个月的0.40±0.29。辟谷后2个月,“代谢”“糖合成及代谢”“非基本氨基酸代谢”“代谢性疾病”功能菌群丰度均明显增高(P<0.05,P<0.01),“信息处理”“膜转运”功能菌群丰度明显降低(P<0.05)。结论辟谷可改变肠道菌群结构与相关功能。Objective To observe the intervention effect of Bigu on gut microbiota.Methods Totally 23 subjects accepted Bigu and health exercises for 5~7 days.The fecal samples were collected 3 days before,3 days and 2 months after the Bigu intervention.DNA extraction and detection,16S r DNA sequence amplification,Mi Seq sequencing,and data analysis were performed in fecal samples.Results Totally 18 subjects finished the Bigu intervention and fecal samples collection for 3 times.Compared with before Bigu intervention,phylum Bacteroidetes,class Bacteroidia and order Bacteroidales increased(P<0.05),family Lachnospiraceae of phylum Firmicutes decreased after 2 months intervention(P<0.05).The ratio of Firmicutes/Bacteroidetes decreased from 0.65±0.67(3 days before intervention)to 0.43±0.25(3 days after intervention),and 0.40±0.29(2 months after intervention).The microbial gene function related to metabolism,glucose synthesis and metabolism,nonessential amino acid metabolism,metabolic diseases increased(P<0.05,P<0.01),while the microbial gene function related to information processing and membrane transport decreased 2 months after intervention(P<0.05).Conclusion Bigu can change bacterial compositions and related function.

关 键 词：辟谷养生术 治未病 肠道菌群 

分 类 号：R212[医药卫生—中医学]