IGF-1和IGF-2在成年牦牛皮肤毛囊生长周期中的表达与定位  

Expression and Localization of IGF-1 and IGF-2 in the Growth Cycle of Hair Follicles in Adult Yak Skin

作　　者：魏鹏强 崔燕[1,2] 余四九 李仕杰[1] 牛静勇 张虔 WEI Pengqiang;CUI Yan;YU Sijiu;LI Shijie;NIU Jingyong;ZHANG Qian(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu 730070;Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep&Goat,Lanzhou,Gansu 730070;Animal Disease Control and Prevention Center of Tianzhu Tibetan Autonomous County,Tianzhu,Gansu 733200)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]甘肃省牛羊胚胎工程研究中心,甘肃兰州730070 [3]天祝县动物疫病预防控制中心,甘肃天祝733200

出　　处：《核农学报》2022年第10期1929-1936,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(31972634)。

摘　　要：为探究胰岛素样生长因子1(IGF-1)和胰岛素样生长因子2(IGF-2)对成年牦牛皮肤毛囊生长周期转换的影响,本研究采集成年牦牛毛囊生长期、退行期和休止期的皮肤,通过实时荧光定量PCR(qRT-PCR)、蛋白免疫印迹(Western blot)和免疫组织化学(IHC)方法检测IGF-1和IGF-2在毛囊生长周期过程中皮肤的表达与定位。qRT-PCR结果显示,IGF-1和IGF-2 mRNA转录水平均在毛囊生长期皮肤中最高,在休止期皮肤中最低,且在各个时期之间差异显著(P<0.05)。Western blot结果表明,IGF-1和IGF-2蛋白表达量均在毛囊生长期皮肤中最高,且显著高于毛囊退行期和休止期皮肤(P<0.05)。IHC结果显示,IGF-1和IGF-2在毛囊生长期、退行期和休止期皮肤中表达位置基本相同,主要定位于皮肤表皮、毛囊外根鞘和毛母质细胞。综上所述,IGF-1和IGF-2都参与毛囊生长周期的调控,并具有协同作用的关系,且该过程可能通过影响上皮细胞的活性来促进毛囊的生长。本研究结果为进一步探讨IGF-1和IGF-2在牦牛毛囊周期循环中的调控机制提供了理论依据。To explore the effects of insulin-like growth factor 1(IGF-1)and insulin-like growth factor 2(IGF-2)on the growth cycle transition of hair follicles in adult yak,the skin samples of adult yak hair follicles were collected at anagen,catagen and telogen.Expression and location of IGF-1 and IGF-2 in the skin during the hair follicle growth cycle were detected by Real-time fluorescence quantitative PCR,western blot and immunohistochemistry.The results of qRT-PCR showed that the transcriptional levels of IGF-1 and IGF-2 mRNA were the highest in anagen and the lowest in telogen,and there were significant differences among different stages(P<0.05).The results of the western blot showed that the protein expression of IGF-1 and IGF-2 was the highest in the skin at anagen,which was significantly higher than that in the skin at catagen and telogen(P<0.05).IHC results showed that IGF-1 and IGF-2 were expressed basically the same in the skin of hair follicle at anagen,catagen and telogen,mainly located in the epidermis,outer root sheath and hair matrix.The results suggest that both IGF-1 and IGF-2 are involved in the coordinate regulation of hair follicle growth cycle,and this process may promote the growth of hair follicles by affecting the activity of epithelial cells.The results of this study provide a theoretical basis for further exploring the regulation mechanism of IGF-1 and IGF-2 on yak hair follicle cycle.

关 键 词：成年牦牛 毛囊周期 胰岛素样生长因子1(IGF-1) 胰岛素样生长因子2(IGF-2) 

分 类 号：S823.85[农业科学—畜牧学]