基于SNP标记的504份番茄种质资源遗传多样性分析  

Genetic Diversity Analysis of 504 Tomato Germplasm Resources Based on SNP Markers

作　　者：宋丽娜 王晓敏[1,2,3,4] 刘文娟 杨宏[1] 付金军[5] 胡新华 高艳明 李建设 SONG Lina;WANG Xiaomin;LIU Wenjuan;YANG Hong;FU Jinjun;HU Xinhua;GAO Yanming;LI Jianshe(School of Agriculture,Ningxia University,Yinchuan,Ningxia 750021;Ningxia Modern Facility Horticulture Engineering Technology Research Center,Yinchuan,Ningxia 750021;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan,Ningxia 750021;Ningxia Facility Horticulture(Ningxia University)Technology Innovation Center,Yinchuan,Ningxia 750021;Ningxia Jufeng Seedling Limited Liability Company,Yinchuan,Ningxia 750021)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏现代设施园艺工程技术研究中心,宁夏银川750021 [3]宁夏优势特色作物现代分子育种重点实验室,宁夏银川750021 [4]宁夏设施园艺(宁夏大学)技术创新中心,宁夏银川750021 [5]宁夏巨丰种苗有限责任公司,宁夏银川750021

出　　处：《核农学报》2022年第12期2366-2373,共8页Journal of Nuclear Agricultural Sciences

基　　金：宁夏回族自治区农业特色优势产业育种专项(NXNYYZ20200104);宁夏回族自治区重点研发计划(重大)重点项目(2019BBF02022)。

摘　　要：为深入了解番茄种质资源的遗传多样性,运用竞争性等位基因特异性PCR(KASP)技术,利用前期筛选出的60对多态性较高的单核苷酸多态性标记(SNP),对收集的504份番茄种质资源进行遗传多样性分析、聚类分析、主成分分析及群体结构分析。结果表明,60个SNP分子标记共检测到181个等位基因,基因多样性平均值为0.450,期望杂合率(He)平均值为0.069,多态性信息值(PIC)变化范围为0.171~0.583,平均值为0.381。在遗传距离为0.36时,504份番茄材料被划分为7个类群,各材料间的平均遗传距离为0.62;根据主成分分析结果将群体分为3个类群;基于SNP标记对参试材料进行群体结构分析,在K=3时,504份番茄种质资源被划分为3类。本研究筛选出的60对SNP标记的多态性为中度偏高,番茄种质资源遗传多样性较丰富,可为后续宁夏地区番茄的核心种质构建及种质资源的有效利用提供理论依据。In order to understand the genetic diversity of tomato germplasm resources,using kompetitive allele specific PCR(KASP)technology,60 pairs of single nucleotide polymorphism(SNP)with high polymorphism were screened in the early stage to analyze the genetic diversity,cluster,principal component and population structure of 504 tomato germplasm resources.The results showed that a total of 181 alleles were detected with 60 SNP markers,the average value of gene diversity was 0.450,the average value of expected heterozygosity(He)was 0.069,and the polymorphism information value(PIC)ranged from 0.171 to 0.583,with an average value of 0.381.Based on clustering,504 tomato materials were divided into 7 groups when the genetic distance was 0.36,and the average genetic distance between each material was 0.62;The population was divided into three groups by principal component analysis;and according to population structure analysis,504 tomato germplasm resources were divided into three categories when K=3.The results showed that the polymorphism of 60 pairs of SNP markers screened in this study is moderately high,and the genetic diversity of tomato germplasm resources is rich,which could provide a theoretical basis for the construction of tomato core germplasm and the effective utilization of germplasm resources in Ningxia.

关 键 词：SNP标记 KASP技术 番茄 种质资源 遗传多样性 

分 类 号：S641.2[农业科学—蔬菜学]