压电陶瓷扫描式双光子内窥成像技术研究进展  

Research Advances in Piezoelectric Ceramic Scanning Two-Photon Endomicroscopy Technology

作　　者：王聪昊 张秀磊[2] 崔皓东 马健睿 吴润龙 刘惠兰[1,4] 王爱民 冯丽爽[1,4] Wang Conghao;Zhang Xiulei;Cui Haodong;Ma Jianrui;Wu Runlong;Liu Huilan;Wang Aimin;Feng Lishuang(School of Instrumentation and Optoelectronic Engineering,Beihang University,Beijing 100191,China;School of Automation Science and Electrical Engineering,Beihang University,Beijing 100191,China;State Key Laboratory of Membrane Biology,Institute of Molecular Medicine,Peking University,Beijing 100871,China;Key Laboratory of Precision Opto-Mechatronics Technology(Ministry of Education),Beihang University,Beijing 100191,China;State Key Laboratory of Advanced Optical Communication System and Networks,School of Electronics,Peking University,Beijing 100871,China)

机构地区：[1]北京航空航天大学仪器科学与光电工程学院,北京100191 [2]北京航空航天大学自动化科学与电气工程学院,北京100191 [3]北京大学分子医学研究所膜生物学国家重点实验室,北京100871 [4]北京航空航天大学精密光机电一体化技术教育部重点实验室,北京100191 [5]北京大学电子学院区域光纤通信网与新型光通信系统国家重点实验室,北京100871

出　　处：《中国激光》2022年第19期226-239,共14页Chinese Journal of Lasers

基　　金：国家重点研发计划(2020YFB1312802);国家自然科学基金(61973019,31830036,61975002)。

摘　　要：双光子内窥成像技术是一种基于双光子激发原理的新型内窥成像技术,具有光学层析能力、穿透深度深、光毒性小、无标记成像等技术优势,可以同时实现细胞结构成像和功能成像,在生命科学、临床医学等领域具有巨大的发展潜力。在近十几年的发展中,压电陶瓷扫描式双光子内窥成像技术不断取得突破,并在生物医学成像领域实现了新应用。本文对压电陶瓷扫描式双光子内窥成像技术及其在国内外的研究进展进行了总结,并介绍了该技术在生物医学成像领域的应用。Significance The incidence and mortality rates of digestive tract cancers are rising quickly globally,greatly endangering human life and health.Most digestive tract tumors come from precancerous lesions,and the development of early cancer detection and diagnosis technology is crucial to improving people's health.To date,histopathological examination is still the"gold standard"for the clinical diagnosis of cancer,but this method has limitations,such as timeconsuming and in vitro detection.Additionally,while biopsy sampling can examine the pathological characteristics of the suspected lesion area at the cellular scale,it cannot achieve full coverage of the suspected lesion area,so there is a certain risk of missed detection and false detection.Therefore,there is an urgent need to develop real-time,in vivo,in situ histological diagnostic techniques at the cellular scale to achieve early diagnosis of GI(gastrointestinal)cancers.Two-photon endomicroscopy is a new type of endomicroscopic imaging technology based on the principle of twophoton excitation,with the technical advantages of optical-sectioning capability,deep penetration,low phototoxicity,and label-free imaging.This technique can realize structural imaging and functional imaging,which has great potential for applications in life science and clinical medicine.Piezoelectric ceramic scanning two-photon endomicroscopy is the current preferred solution for two-photon endomicroscopy imaging technology.In recent years,this technique has achieved technological breakthroughs and new applications.This paper summarizes piezoelectric ceramic scanning two-photon endomicroscopic imaging technology and the research progress and introduces its application in the field of biomedical imaging.Process Section 2 introduces three typical two-photon endomicroscopy systems:fiber bundle proximal scanning scheme,MEMS distal scanning scheme,and piezoelectric ceramic-driven fiber distal scanning scheme(Fig.1).Subsequently,the system structure and breakthroughs in core device technology

关 键 词：医用光学 双光子成像 内窥镜 双包层光纤 微型显微物镜 压电陶瓷扫描器 

分 类 号：Q-334[生物学]