基于荧光方法的肿瘤标志物检测研究进展  

Progress in Tumor Biomarker Detection Based on Fluorescence Method

作　　者：董彪 郭丽华 刘大勇 王宇达 刘伟 杨瑞 何海涛[2] 孙娇[2] Dong Biao;Guo Lihua;Liu Dayong;Wang Yuda;Liu Wei;Yang Rui;He Haitao;Sun Jiao(State Key Laboratory of Integrated Optoelectronics,College of Electronic Science and Engineering,Jilin University,Changchun 130012,Jilin,China;Department of Cell Biology,College of Basic Medical Sciences,Jilin University,Changchun 130021,Jilin,China)

机构地区：[1]吉林大学电子科学与工程学院集成光电子学国家重点实验室,吉林长春130012 [2]吉林大学基础医学院细胞生物学系,吉林长春130021

出　　处：《中国激光》2022年第20期17-38,共22页Chinese Journal of Lasers

基　　金：国家重点研发计划(2016YFC0207101);国家自然科学基金(81602659,61775080);吉林省教育厅“十三五”科学技术项目(JJKH20190101KJ);吉林省自然科学基金(20170101170JC)。

摘　　要：癌症的早期发现可以显著降低死亡率,所以癌症的早期检测与诊断已成为生物医学领域最关注的研究方向之一。肿瘤生物标志物在癌症风险评估、筛查诊断、预测治疗、预后监测等方面具有重要作用和巨大的应用潜力。目前,生物标志物的检测方法主要有放射免疫分析法、酶联免疫分析法、质谱法、光学方法、纳米技术、电化学法、微流控技术等。荧光方法具有高灵敏度、高分辨率、操作简便等特点,在无创检测和快速检测上具有重要的应用前景。本文介绍了荧光方法在肿瘤生物标志物检测方面的最新进展,从循环肿瘤细胞、循环肿瘤DNA、外泌体及肿瘤标志物蛋白(癌胚抗原、甲胎蛋白、前列腺特异性抗原)等方面进行了综述,总结了最新的荧光检测技术,对基于液体活检技术的肿瘤早期检测研究具有指导意义。Significance Cancer,characterized by abnormal cell proliferation,is one of the most important chronic and complex diseases.Not only is it a serious threat to people’s health and life,but also it has been a leading cause of death in every country in the world for a long time.It has caused huge loss to society,including the negative influence on the emotions and economies of patients and their families,the burden on medical resources and treatment investments,and the loss of human resources.The cancer survival rate is usually low,which may be related to a lack of timely and effective detection,which leads to late diagnosis and missed opportunities for targeted and standardized treatment.Early detection and effective diagnosis of cancer can significantly reduce the death rates of patients through early and effective prevention and treatment.Tumor markers are defined as substances that are synthesized and released by tumor cells themselves or that are produced by the body in response to the development and proliferation of tumors,and which can be identified in blood,urine,saliva,tissue,and other body fluids.Detection of tumor markers is an important indicator for cancer diagnosis,treatment,and efficient monitoring.It complements other clinical examination methods,such as imaging,endoscopic,and pathological examinations.The early detection of tumor markers has the advantages of being minimally or noninvasive,rapid,and convenient,and it has great application potential in cancer screening,diagnosis,treatment,prognosis,and other aspects.In the past few decades,advances in the early detection of cancer have led cancer research to a minted stage.Based on the specific recognition of intracellular and extracellular biomarkers,several promising detection methods have been developed,including the polymerase chain reaction,enzyme-linked immunosorbent assay,electrophoresis,surface plasmon resonance,surface-enhanced Raman spectroscopy,electrochemical sensing,mass spectrometry,flow cytometry,and other technologies.However,some o

关 键 词：医用光学 荧光 肿瘤生物标志物 纳米材料 液体活检 

分 类 号：O436[机械工程—光学工程]