飞秒激光焊接离体皮肤组织工艺与性能试验研究  

Technique and Properties of Femtosecond Laser Welding In Vitro Skin Tissue

作　　者：宋紫燕 黄俊[1] 李英哲 李骐彤 夏胜男 王克鸿[1] 芮云峰[2] Song Ziyan;Huang Jun;Li Yingzhe;Li Qitong;Xia Shengnan;Wang Kehong;Rui Yunfeng(School of Materials Science and Engineering,Nanjing University of Science and Technology,Nanjing 210094,Jiangsu,China;Orthopedics,Zhongda Hospital,Southeast University,Nanjing 210009,Jiangsu,China)

机构地区：[1]南京理工大学材料科学与工程学院,江苏南京210094 [2]东南大学附属中大医院骨科,江苏南京210009

出　　处：《中国激光》2022年第20期53-61,共9页Chinese Journal of Lasers

摘　　要：为了获得飞秒激光工艺参数对生物组织融合效果的影响规律,基于单因子试验法,分析了激光功率、离焦量等工艺参数对离体皮肤组织融合形貌、切口抗拉强度及热损伤的影响规律。结果表明,激光功率及扫描速度对焊后离体皮肤组织外观形貌及切口抗拉强度具有重要影响,激光功率对组织热损伤具有决定性影响;降低激光功率及扫描速度,采用多次重复扫描方法,焊后离体皮肤组织的切口抗拉强度较大;在保证一定连接强度的条件下,焊接后组织热损伤参数在10^(-3)数量级左右。在此基础上,对工艺参数进行优化,结果表明,在该工艺条件下,组织切口可实现全层融合,外观形貌良好,热损伤低于采用连续激光得到同等抗拉强度时组织所产生的热损伤。Objective Laser welding of biological tissues is a noncontact suture technology.Compared to the traditional contact needle and thread suture,it has several advantages,such as simpler operation,faster speed,quicker postoperative recovery,and comparatively insignificant scars.Initial success has already been achieved in the welding of the skin,blood vessels,and lung tissue.As an ultrafast pulsed laser,the femtosecond laser has an extremely high peak power and an exceptionally short action time,that helps to avoid the linear absorption,transfer,and diffusion of energy to a large extent.Thus,it has been widely used in the medical field.However,there are only a few reports on the technology of using femtosecond laser for welding biological tissues.The mechanism of interaction between the femtosecond laser and tissue is not yet clear,and the influence of laser process parameters on the incision fusion effect of isolated skin tissue needs to be further studied.Therefore,in this study,we analyze the effects of laser power,defocus amount,and other process parameters on the fusion morphology of in vitro skin tissue,its incision tensile strength,and thermal damage by a mono-factorial experimental method.All the process parameters of the femtosecond laser are also optimized.We believe that our experiment and results will be helpful in determining the effect of femtosecond laser parameters on the fusion effect of biological tissue and promote further research on the laser welding of biological tissue.Methods This study adopts the mono-factorial experiment method considering that there are many factors affecting tissue fusion,while retaining the other process parameters.The four factors of the femtosecond laser power,defocus amount,scanning speed,and number of scannings are varied and each factor is set to 3-4levels.Femtosecond laserwelding experiments are performed onin vitro pigskin.Subsequently,using a tensile force meter,a tensile strength test is conducted to obtain the tensile strength of the incision.During the welding

关 键 词：医用光学 组织焊接 激光材料加工 热效应 组织性能 参数优化 

分 类 号：TN249[电子电信—物理电子学]