基于OCT的昆虫心脏功能参数自动检测定量分析方法  

Automatic Detection and Quantitative Analysis of Insect Cardiac Function Parameters Using OCT

作　　者：王秀丽[1,2] 杜若瑄 姚晓天 苏亚 崔省伟 郝鹏[1,2] 杨丽君 段兵兵 Wang Xiuli;Du Ruoxuan;XSteve Yao;Su Ya;Cui Shengwei;Hao Peng;Yang Lijun;Duan Bingbing(College of Physics Science&Technology,Hebei University,Baoding 071002,Hebei,China;Hebei Provincial Center for Optical Sensing Innovations,Baoding 071002,Hebei,China;Institute of Life Science and Green Development,Hebei University,Baoding 071002,Hebei,China)

机构地区：[1]河北大学物理科学与技术学院,河北保定071002 [2]河北省光学感知技术创新中心,河北保定071002 [3]河北大学生命科学与绿色发展研究院,河北保定071002

出　　处：《中国激光》2022年第20期78-85,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(62105091);河北省重点研发计划(19212109D,20542201D);河北省自然科学基金(F2020201041,F2021201016);河北省高等学校科学技术研究基金项目(QN2019035);河北大学“新医科”教学改革项目(2020XYKY08)。

摘　　要：心血管疾病是一种严重威胁人类健康的常见病。目前常用手段之一是以昆虫为模式生物,利用RNAi等技术抑制特定基因表达,通过表型检测或监测其心脏功能参数等来开展此类疾病致病机理的研究。光学相干层析技术(Optical Coherence Tomography,OCT)因其无创、实时、高分辨率等特点,被成功应用于昆虫等模式生物的心脏功能检测方面。但目前的检测计算方法仍存在效率低、对图像质量要求高、参数测量不准确等问题,尤其不适用于生物实验中大样本量的检测分析。提出一种基于OCT的昆虫心脏功能参数高速自动检测定量分析计算方法。该算法结合OCT心脏M-Mode图,采用人机交互的模式确定种子点所在的位置,通过自动分割图像、划分目标区域等一系列处理,可以快速准确地测量心脏舒张末期直径(EDD)、收缩末期直径(ESD)、舒张末期面积(EDA)、收缩末期面积(ESA)及心率(HR)等心脏功能参数。该方法有助于提升高通量生物样本检测中致病基因的筛查和分析效率,对以昆虫为模式生物的心血管疾病研究具有重要的价值。Objective Cardiovascular is one of the major diseases that threatens human health,and the prevalence of cardiovascular disease in China continues to grow.Therefore,it is important to select an appropriate model organism to understand the development of the heart.Locust has the characteristics of easy operation,strong plasticity,and short development cycle as well as the similar gene regulation mechanism with human beings in the process of cardiac development,therefore it becomes a useful candidate for studying the cardiac function and for the pathological gene analysis.Researchers have proposed a variety of methods to evaluate the heart function of insects,such as multi-sensor electrocardiogram,atomic force microscope monitoring,and electrical stress method.However,these methods are invasive and cannot monitor the same living body continuously.Therefore,a method which can monitor the heart development and screen the phenotypic variation of insects or other model organisms non-invasively is more desiderated.Fortunately,optical coherence tomography(OCT),widely used in biomedical detection because of its noninvasiveness,real-time,and high resolution,can be used to detect the internal structures of biological tissues and other non-uniform scatterers.Therefore,it is a more suitable tool to monitor the embryonic heart development of a locust.In addition,the measurement of cardiac function parameters(such as heart rate)still needs to be calculated manually by the M-Mode diagram,which is not only time-consuming but also prone to errors.Therefore,a high efficiency automatic detection algorithm is a critical issue to be solved urgently in the high-throughput screening and phenotypic analysis of model biological pathogenic genes.Methods Using a locust as the model organism,in our previous works we have monitored the embryo development and screened the phenotypic variation caused by the RNAi technology.Here,a new method is proposed to automatically and quickly calculate the insect heart function parameters,such as end diasto

关 键 词：生物技术 光学相干层析 昆虫 模式生物 心脏功能参数 

分 类 号：TN247[电子电信—物理电子学]