基于数字全息显微层析的癌细胞空泡化成像研究  

Cancer Cell Vacuolization Imaging Based on Digital Holographic Microscopy Tomography

作　　者：刘雅坤 肖文[1] 车蕾平 李胤嘉 肖溪 王皓[2] 潘锋[1] Liu Yakun;Xiao Wen;Che Leiping;Li Yinjia;Xiao Xi;Wang Hao;Pan Feng(Key Laboratory of Precision Opto-Mechatronics Technology,School of Instrumentation&Optoelectronic Engineering,Beihang University,Beijing 100191,China;Department of Radiation Oncology,Peking University Third Hospital,Beijing 100191,China;Medical Research Center,Peking University Third Hospital,Beijing 100191,China)

机构地区：[1]北京航空航天大学仪器科学与光电工程学院精密光机电一体化技术重点实验室,北京100191 [2]北京大学第三医院肿瘤放射治疗科,北京100191 [3]北京大学第三医院基础医学研究中心,北京100191

出　　处：《中国激光》2022年第20期146-156,共11页Chinese Journal of Lasers

基　　金：北京市自然科学基金(M22017);北京大学第三医院创新转化基金(BYSYZHKC2021113)。

摘　　要：癌症作为一种恶性化程度和致死率很高的疾病,是世界各国共同面对的难题。研究癌细胞的死亡过程,对于研究癌症的病理学机理以及探究有效的癌症治疗方法具有重要意义。本团队采用数字全息显微层析成像技术,对膀胱癌细胞内部的空泡进行了全息记录和衍射再现,获得了癌细胞在不同角度下的复光场数据;然后采用衍射层析重建算法结合非负约束条件,层析重构了癌细胞内部空泡的三维形态和空间位置,证实了数字全息显微层析技术在癌细胞内部空泡三维成像方面的优势和潜力。这一成像方式在研究癌细胞类凋亡过程中的形态变化以及探索新的癌症治疗策略等方面具有重要的应用价值。Objective Cancer is a disease caused by the uncontrolled growth and division of malignant cancer cells.Since the 21st century,the incidence and mortality of cancer have been increasing rapidly worldwide,making it a medical problem that affects countries worldwide.The pathological mechanism of cancer and the study of various therapeutics based on inducing cancer cell death both greatly benefit from research on the morphology and function of cancer cells at the singlecell level,particularly research on the process of cancer cell death.Digital holographic microscopy,aquantitative phase imaging technique,offers a nondestructive,unlabeled,and noncontact quantitative measurement tool for biological research.It can also provide nondestructive quantitative imaging of living cells.In this paper,digital holographic tomography was used in the three-dimensional quantitative detection of bladder cancer cell vacuolation.This work can broaden the field of application for digital holographic tomography in the biomedical industry,offer new perspectives on how to study the morphological changes that occur during cancer cell apoptosis,and investigate potential new cancer treatment approaches.Methods This study focuses on the vacuolar structure of cancer cells existing in the process of paraptosis.First,the hologram of bladder cancer cells with vacuoles inside was obtained using digital holographic microscopy.Then,the amplitude and phase of cells were obtained by filtering,digital focusing,angular spectrum propagation,and phase unwrapping.Their three-dimensional morphology and spatial locations were reconstructed using the diffraction tomography reconstruction algorithm combined with nonnegative constraints.Finally,morphological parameters such as the surface and volume of the vacuoles were calculated according to the number of pixels in the image.Results and Discussions Digital holographic tomography was used in this study to produce three-dimensional reconstruction results of four bladder cancer cells with vacuoles(Figure 8).Four

关 键 词：医用光学 全息 显微成像 层析技术 癌细胞空泡化 

分 类 号：R318.51[医药卫生—生物医学工程]