玉米株高QTL的定位及主效QTL的确认  

Mapping of Plant Height QTL and Confirmation of a Major QTL in Maize

作　　者：高歌 杨媛 郑军[2] 张红伟[2] GAO Ge;YANG Yuan;ZHENG Jun;ZHANG Hongwei(College of Agronomy,Jilin Agricultural University,Changchun,Jilin 130118;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;College of Agronomy,China Agricultural University,Beijing 100193)

机构地区：[1]吉林农业大学农学院,吉林长春130118 [2]中国农业科学院作物科学研究所,北京100081 [3]中国农业大学农学院,北京100193

出　　处：《核农学报》2022年第8期1530-1536,共7页Journal of Nuclear Agricultural Sciences

基　　金：中国农业科学院创新工程(CAAS-ZDRW202004)。

摘　　要：为了探索玉米株高的遗传机制,定位玉米株高的数量性状位点(QTL),本研究以玉米自交系PH4CV为轮回亲本,以郑58为供体亲本,构建BC_(1)F_(3:4)分离群体,在4个环境下对该群体进行玉米株高表型鉴定。表型分析结果表明,基因型之间差异极显著,且不同环境之间的株高相关性极显著,说明不同环境之间的株高变异具有共同的遗传基础。利用包含5.5万个单核苷酸多态性标记(SNPs)的基因芯片进行基因型鉴定,并结合基因型和表型数据进行全基因组关联分析。在错误发现率(FDR)为0.05时,检测到10个显著性SNPs,这些显著性SNPs主要位于第2号染色体上,-log_(10)(P)值最大的标记为Chr2_194690794。利用线性回归模型对显著SNPs进行表型贡献率及效应分析,发现位于第2号染色体的标记Chr2_194690794效应值最大,贡献率最高,来源于PH4CV的基因型的正效应。利用BC_(1)F_(5:6)群体进行基因型和表型鉴定,进一步确认了标记Chr2_194690794与株高QTL的连锁关系,表明在第2号染色体上存在1个控制株高的QTL。本研究为玉米株高QTL的精细定位奠定了基础。In order to dissect the genetic mechanism of plant height and map the quantitative trait locus/loci(QTL)in maize,we used maize inbred lines PH4CV and Zheng 58 as the recurrent and donor parents respectively to construct a BC_(1)F_(3:4) segregation population.Plant height in this population was evaluated for in four environments.Phenotypic analysis showed that the genotypic differences of plant height was great,and the correlations of plant heights evaluated in the four environments were strong,indicating that plant height in different environments had common genetic basis.The population was genotyped using gene chips containing 55000 SNPs.By genome-wide association analysis,we detected ten significant single nucleotide polymorphisms(SNPs)with the false positive rate(FDR)at 0.05,and identified a significant SNP hotspot on chromosome 2.The SNP Chr2_194690794 on chromosome 2 had the highest-log_(10)(P)value.Linear regression analysis revealed that Chr2_194690794 had the largest additive effect and explained the largest phenotypic variation.For Chr2_194690794,the genotype derived from PH4CV had a positive contribution to maize plant height.Using a BC_(1)F_(5:6) population,we further verified the linkage between Chr2_194690794 and plant height,proving that there was a real QTL on chromosome 2.This study laid a foundation for fine mapping of plant height QTLs.

关 键 词：玉米 株高 关联分析 QTL 

分 类 号：S513[农业科学—作物学]