麦冬、大黄饮片电子束辐照灭菌工艺研究  

Study on the Decontamination Process of Decoction Pieces of Ophiopogon japonicus and Rheum palmatum L. by Electron Beam Irradiation

作　　者：王钢 王丹[1] 何毅 付孟 唐艺文 青川 高鹏 黄敏[2,3] WANG Gang;WANG Dan;HE Yi;FU Meng;TANG Yiwen;QING Chuan;GAO Peng;HUANG Min(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010;Sichuan Institute Atomic Energy,Chengdu,Sichuan 610101;Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu,Sichuan 610101)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川省原子能研究院,四川成都610101 [3]辐照保藏四川省重点实验室,四川成都610101

出　　处：《核农学报》2022年第8期1579-1588,共10页Journal of Nuclear Agricultural Sciences

基　　金：四川省重大科技专项项目(2019ZDZX0003)。

摘　　要：为了研究电子束辐照处理对麦冬、大黄饮片灭菌的工艺及效果,以麦冬、大黄饮片为材料,研究不同剂量(0、2、4、6、8 kGy)电子束辐照对其微生物总数、理化指标、指纹图谱相似度及有效成分含量的影响。根据不同堆码厚度、辐照方式计算剂量不均匀度,优化麦冬、大黄饮片的电子束辐照加工工艺。结果表明,电子束辐照能明显降低麦冬、大黄饮片中的微生物数量;电子束辐照对麦冬饮片水分、水溶性浸出物及总灰分含量的变化无显著影响(P>0.05);电子束辐照有利于提高大黄饮片水溶性浸出物含量,对其干燥失重、总灰分变化无显著影响(P>0.05);辐照前后麦冬、大黄饮片的指纹图谱相似度均大于0.99,相对保留时间(RSD)均小于0.5%;不同剂量电子束辐照对麦冬、大黄饮片有效成分含量均无显著影响(P>0.05);剂量不均匀度与产品堆码厚度及辐照方式密切相关。综上,麦冬饮片适宜辐照剂量为2~8 kGy,大黄饮片适宜剂量为4~8 kGy,此剂量范围对麦冬、大黄饮片整体质量无显著影响。单面辐照时,麦冬饮片适宜堆码厚度为4 cm,大黄饮片适宜堆码厚度为4~6 cm;双面辐照时,麦冬饮片适宜堆码厚度为10 cm,大黄饮片适宜堆码厚度为14 cm。本研究为麦冬、大黄加工贮藏及电子束辐照在中药中的应用提供了理论依据与技术支持。In order to study the decontamination process and effect of electron beam irradiation on the decoction pieces of Ophiopogon japonicus and Rheum palmatum L.,the effects of different doses(0,2,4,6,and 8 kGy)of electron beam irradiation on the total number of microorganisms,physical and chemical indexes,fingerprint similarity,and active components were analyzed.The uniformity of absorbed was calculated with different stacking thickness and irradiation methods,and the decontamination process of decoction pieces of Ophiopogon japonicus and Rheum palmatum L.by electron beam irradiation was established.The results showed that electron beam irradiation could significantly reduce the number of microorganisms,electron beam irradiation had no significant effect on the moisture change,water-soluble leachate and total ash of decoction pieces of Ophiopogon japonicus(P>0.05).Electron beam irradiation could be beneficial to improve the content of water-soluble leachate in decoction pieces of Rheum palmatum L.,and had no significant effect on dry weightlessness and total ash change(P>0.05).The similarity calculation of chromatography fingerprint of decoction pieces of Ophiopogon japonicus and Rheum palmatum L.pre-and post-irradiation was greater than 0.99,and the RSD of relative retention times was less than 0.05%.Different irradiation doses had no significant effect on the content of active components in Ophiopogon japonicus and Rheum palmatum L.(P>0.05).The uniformity of absorbed is closely related to the product stack thickness and irradiation methods.To sum up,the suitable dose of decoction pieces of Ophiopogon japonicus was 2 to 8 kGy,and the appropriate dose of decoction pieces of Rheum palmatum L.was 4 to 8 kGy,which had no significant effect on the overall quality of decoction pieces of Ophiopogon japonicus and Rheum palmatum L.under the dose range.When irradiated on one side,the suitable stacking thickness of Ophiopogon japonicus and Rheum palmatum L.was 4 cm and 6 cm respectively.When irradiated on both sides,the suitab

关 键 词：麦冬饮片 大黄饮片 电子束 辐照灭菌 工艺 

分 类 号：R283.6[医药卫生—中药学]