山茱萸果实化学成分及其抗阿尔茨海默症活性研究  

Chemical constituents from fruits of Cornus officinalis and their anti-Alzheimer’s disease activity

作　　者：杨梦[1,2] 郝志友 周诗琪[1,2] 曹兵 朱登辉 杨颖 魏俊俊 陶思琦 张博文 郑晓珂 冯卫生 YANG Meng;HAO Zhi-you;ZHOU Shi-qi;CAO Bing;ZHU Deng-hui;YANG Ying;WEI Jun-jun;TAO Si-qi;ZHANG Bo-wen;ZHENG Xiao-ke;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Engineering and Technology Research Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术研究中心,河南郑州450046

出　　处：《药学学报》2022年第12期3608-3615,共8页Acta Pharmaceutica Sinica

基　　金：中央引导地方科技发展专项资金([2016]149);河南省科技攻关项目(212102311091);河南省高层次人才特殊支持计划(ZYQR201810080)。

摘　　要：采用Toyopearl HW-40C、Sephadex LH-20、硅胶和半制备液相等多种色谱学技术从山茱萸果实中分离得到15个化合物。根据理化性质与波谱数据鉴定了其结构,分别为新木脂素A(1)、咖啡酸(2)、反式对羟基桂皮酸(3)、马栗树皮素(4)、东莨菪素(5)、benzyl-7-O-β-D-glucopyranoside(6)、它乔糖苷(7)、6-O-(4-hydroxybenzoyl)arbutin(8)、2-(3′,4′-二羟苯基)-1,3-胡椒环-5-醛(9)、(-)-松脂素-4-O-β-D-吡喃葡萄糖苷(10)、(7S,8R)-二氢去氢二愈创木基醇-9-O-β-D-吡喃葡萄糖苷(11)、(7S,8R)-二氢去氢二松柏醇-9′-O-β-D-吡喃葡萄糖苷(12)、(+)-南烛木树脂酚(13)、(+)-异落叶松树脂醇-9-O-β-D-吡喃葡萄糖苷(14)、异落叶松树脂醇-9′-O-β-D-吡喃葡萄糖苷(15)。化合物1为新化合物,命名为新木脂素A,化合物6~9和14首次从山茱萸中分离得到。化合物2、3和15可以有效缓解Aβ25-35诱导的PC12细胞损伤,表明具有潜在的抗阿尔茨海默症活性。Fifteen compounds were isolated from fruits of Cornus officinalis by various chromatographic techniques such as Toyopearl HW-40C,Sephadex LH-20,silica gel,and the semi-preparative HPLC.Their chemical structures were identified by analysis of physicochemical properties and spectral data,and determined as neolignan A(1),caffeic acid(2),trans-p-hydroxy cinnamic acid(3),esculetin(4),scopoletin(5),benzyl-7-O-β-D-glucopyranoside(6),tachioside(7),6-O-(4-hydroxybenzoyl) arbutin(8),2-(3′,4′-dihydroxyphenyl)-1,3-benzodioxole-5-carboxaldehyde(9),(-)-pinoresinol-4-O-β-D-glucopyranoside(10),(7S,8R)-dihydrodehydrodiconiferyl alcohol-9-O-β-D-glucopyranoside(11),(7S,8R)-dihydrodehydrodiconiferyl alcohol-9′-O-β-D-glucopyranoside(12),(+)-lyoniresinol(13),(+)-isolariciresinol-9-O-β-D-glucopyranoside(14),and isolariciresinol-9′-O-β-D-glucopyranoside(15).Compound 1 was a new compound and named as neolignan A,and compounds 6-9 and 14 were isolated from Cornus officinalis for the first time.Compounds 2,3 and 15 efficiently alleviated the PC12 cells injury induced by Aβ25-35,suggesting their potential anti-Alzheimer’s disease activity.

关 键 词：山茱萸 化学成分 木脂素苷 新木脂素A 抗阿尔茨海默症活性 

分 类 号：R284[医药卫生—中药学]