辣椒根腐病菌拮抗链霉菌的筛选及16S rRNA基因系统发育分析  

Antagonistic Streptomyces Against Pepper Root Rot Pathogen(Fusarium oxysporum):Screening and Phylogenetic Analysis of 16S rRNA Gene Sequence

作　　者：努尔夏提·努尔买买提 李焕宇 杨成德 Nuerxiati·Nuermaimaiti;LI Huanyu;YANG Chengde(College of Plant Protection,Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070)

机构地区：[1]甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《中国农学通报》2022年第33期116-123,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目“基于形态特征和谱系相合性系统发育物种识别的甘肃省辣椒镰刀菌根部病害病原菌分类”(31960009)。

摘　　要：为获得对辣椒根腐病菌尖镰孢菌(Fusarium oxysporum)有拮抗作用的链霉菌并明确其抑菌效果与分类情况,以尖镰孢菌为指示菌,采用平板对峙法筛选,结合菌丝生长速率法测定抑菌率,采用16S rRNA基因序列进行聚类分析。初筛获得8株有拮抗作用的链霉菌,对峙培养中对尖镰孢菌的抑菌率为45.18%~62.81%,对其他4种根腐病菌的抑菌率为28.51%~60.00%。菌株ZY12.53b的拮抗作用最好,对5种根腐病菌的抑菌率均大于50%。菌株MDI2.4和ZY12.23a的发酵滤液对三线镰孢菌的拮抗作用最强,抑菌率均大于60%。16S r RNA基因序列系统发育树中,8株链霉菌聚在5个不同的分支中,且其中2个分支很可能为该属潜在的新种。本研究获得链霉菌属8个拮抗菌株,至少包括5个种,其中3个菌株拮抗效果较好,具挖掘利用价值。In order to obtain Streptomyces strains with antagonistic effect on Fusarium oxysporum causing pepper root rot and clarify their antagonistic effect and classification, F. oxysporum was used as the indicator strain to screen by plate confrontation method. The inhibition rate was determined by mycelial growth rate method. Phylogenetic analysis was conducted based on 16S rRNA gene sequence. In this study, eight antagonistic Streptomyces strains were obtained by primary screening. The results of confrontation culture showed that the inhibition rate against F. oxysporum was 45.18%-62.81%, and the inhibition rate against the other four root rot fungi was 28.51%-60.00%. Among them, the strain ZY12.53b showed the best antagonistic effect with more than 50% inhibition rate against five root rot fungi. The fermentation filtrates of strains MDI2.4and ZY12.23a had the highest antagonistic effect on F. tricinctum, and the inhibition rates were both more than60%. In the phylogenetic tree of 16S rRNA gene sequence, the eight antagonistic Streptomyces strains clustered into five different branches, and two of them were likely to be potential new species. In this study, eight antagonistic Streptomyces strains belonging to at least five species were obtained, among which three strains showed satisfied antagonistic effect with the value of potential exploration.

关 键 词：链霉菌 尖镰孢菌 筛选 系统发育分析 

分 类 号：S311[农业科学—作物栽培与耕作技术]