烟草两种质源胞质雄性不育系生化特性比较  

Comparative Analysis of Biochemical Characteristics for Two Sources of CMS-Nta(gla)S and Nta(sua)S

作　　者：彭坚强[1,2] 陈学军 吴兴富[1] 焦芳婵[1] 冯智宇[1] 杨大海 张谊寒[1] 刘天彦[3] 杨林明 PENG Jianqiang;CHEN Xuejun;WU Xingfu;JIAO Fangchan;FENG Zhiyu;YANG Dahai;ZHANG Yihan;LIU Tianyan;YANG Linming(Yunnan Academy of Tobacco Agricultural Sciences,Kunming,Yunnan 650021,China;Yunnan Tobacco Company Baoshan Branch,Baoshan,Yunnan 678000,China;Yunnan Tobacco Company Qujing Branch,Qujing,Yunnan 655000,China)

机构地区：[1]云南省烟草农业科学研究院,云南昆明650021 [2]云南省烟草公司保山市公司,云南保山678000 [3]云南省烟草公司曲靖市公司,云南曲靖655000

出　　处：《作物研究》2022年第6期575-578,584,共5页Crop Research

基　　金：中国烟草总公司科技项目(110201302005);中国烟草总公司云南省公司科技项目(2021530000241017)。

摘　　要：为比较不同质源烟草雄性不育系及其保持系的生化特性差异,以源自粉蓝烟草(Nicotiana glauca)胞质雄性不育系K326[(gla)S]和源自甜烟草(N.suaveolens)胞质雄性不育系K326[(sua)S]及保持系K326(MF K326)为材料,测定生育期过氧化物酶(POD)和超氧化物歧化酶(SOD)活性,在盛花期测定ATP、ADP含量。结果表明:主要生育期内,MF K326的POD及SOD活性均高于(gla)S和(sua)S;成苗期到旺长期(gla)S的POD酶活性低于(sua)S,而从现蕾期到盛花期,(gla)S的POD酶活性高于(sua)S;由成苗期到现蕾期(gla)S的SOD酶活性高于(sua)S;而从现蕾期到盛花期,(gla)S的SOD活性低于(sua)S。盛花期叶片组织中,MF K326、(gla)S、(sua)S的ATP含量分别是其ADP含量的1.72、1.27、1.56倍;MF K326的POD及SOD酶活性均显著高于(gla)S及(sua)S,(gla)S的POD酶活性显著低于(sua)S,而(gla)S与(sua)S间SOD酶活性无显著差异。盛花期花蕾组织中,MF K326、(gla)S、(sua)S的ATP含量分别是其ADP含量的2.02、1.19、1.39倍;MF K326的POD及SOD酶活性均显著高于(gla)S及(sua)S,(gla)S及(sua)S之间则无显著差异。这为进一步开展胞质不育系机理研究及杂种优势利用提供了基础。This paper aimed to compare the biochemical characteristics of two sources of tobacco CMS.In this paper, the two sources of tobacco CMS cutivars, namely the cultivar(gla)S with the cytoplasm from N.glauca in combination with the N.tabacum nucleus, the cultivar(sua)S with the cytoplasm from N.suaveolens in combination with the N.tabacum nucleus and maintainer line was used as materials for biochemical analysis.The enzyme activity of peroxidase(POD) and superoxide dismutase(SOD) in leaves of the materials mentioned above were measured at the main growth stage of tobacco.The comparative analysis for the activity of POD and SOD,the content of ATP and ADP in the leaves and buds was also conducted at the full flowering stage.The enzyme activity of POD and SOD for MF K326 were higher than those of(gla)S and(sua)S at the main growth period.The POD enzyme activity of(gla)S was lower than that of(sua) S,and from budding stage to full flowering stage, the POD enzyme activity of(gla) S was higher than that of(sua) S;The SOD enzyme activity of(gla)S was higher than that of(sua)S;while from budding to full flowering stage, the SOD activity of(gla)S was lower than that of(sua)S.In leaf tissue at full flowering stage, the ATP content of MF K326 was 1.72 times that of its ADP,the ATP content of(gla) S was 1.27 times than that of its ADP,and the content of(sua) S was 1.56 times.The enzyme activity of SOD and POD in MF K326 were significantly higher than those of(gla)S and(sua)S,the POD enzyme activity of(gla)S was significantly lower than that of(sua)S,and no significantly difference for the SOD enzyme activity between(gla)S and(sua)S was observed.In bud tissue at full bloom, the ATP content of MF K326 was 2.02 times that of its ADP,the ATP content of(gla)S was 1.19 times that of its ADP,and the content of(sua)S was 1.39 times.The POD enzyme and SOD enzyme activities of MF K326 were significantly higher than those of(gla)S and(sua)S,and there was no significant difference between(gla)S and(sua)S for POD and SOD enzyme activities.T

关 键 词：烟草 胞质雄性不育系 ATP ADP POD酶 SOD酶 

分 类 号：S572.032[农业科学—烟草工业]