电针足三里对脾气虚模型大鼠骨骼肌线粒体呼吸链复合物活性和ATP含量的影响  

Effects of Electroacupuncture at Zusanli(ST36)on the Activities of Mitochondrial Respiratory Chain Complexes and ATP Content in Skeletal Muscles of Rats with Spleen Qi Deficiency

作　　者：勇入琳 刘路 董佳梓 张立德[2] YONG Rulin;LIU Lu;DONG Jiazi;ZHANG Lide(Zhejiang Chinese Medical University,Hangzhou 310005,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]浙江中医药大学,杭州310005 [2]辽宁中医药大学,沈阳110847

出　　处：《上海针灸杂志》2022年第12期1217-1222,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81603704);浙江省自然科学基金项目(LQ20H270004);浙江省教育厅科研项目(Y201840376);浙江中医药大学中青年科研创新基金项目(KC201919)。

摘　　要：目的观察电针足三里穴对脾气虚模型大鼠骨骼肌线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性以及三磷酸腺苷(ATP)含量的影响。方法将32只雄性SD大鼠随机分为正常组、模型组、足三里组和非穴组,每组8只。采用劳倦过度及饮食不节的复合因素制备大鼠脾气虚证候模型,足三里组予电针双侧足三里穴治疗,非穴组予电针双侧非经非穴点治疗。观察各组大鼠的毛色、进食量和粪便情况,并测量各组大鼠前肢抓力以评估肌肉力量;采用比色法检测大鼠骨骼肌组织三磷酸腺苷(ATP)含量,用酶标法检测大鼠骨骼肌组织线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性。结果造模后,与正常组比较,模型组、足三里组和非穴组大鼠体质量和抓力显著下降(P<0.05),骨骼肌组织线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性及ATP含量明显降低(P<0.05)。与模型组比较,足三里组大鼠体质量略增加、抓力明显增大(P<0.05),骨骼肌组织线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性及ATP含量显著升高(P<0.05);非穴组大鼠上述指标与模型组比较,差异无统计学意义(P>0.05)。结论电针足三里穴可明显提高脾气虚模型大鼠骨骼肌线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性,增加ATP含量,参与线粒体呼吸及能量代谢的调控作用而发挥健脾益气的功效。Objective To observe the effects of electroacupuncture at Zusanli(ST36)on the activities of mitochondrial respiratory chain complexes Ⅰ,Ⅱ,Ⅲ,and Ⅳ and the content of adenosine triphosphate(ATP)in skeletal muscles of rats with spleen Qi deficiency.Method Thirty-two male Sprague-Dawley(SD)rats were randomly assigned to a normal group,a model group,a Zusanli group,and a non-acupoint group,with 8 rats in each group.The spleen-Qi-deficiency rat model was established using exhaustive swimming alongside improper diet.The Zusanli group was intervened by electroacupuncture at Zusanli,and the non-acupoint group received electroacupuncture at spots away from meridians.Hair color,food intake,and stool pattern were observed.The rats’front paw’s grip force was measured to assess muscle strength;the skeletal muscle ATP content was determined using colorimetric assay;the enzyme labeling method was adopted to detect the activities of mitochondrial respiratory chain complexes Ⅰ,Ⅱ,Ⅲ,and Ⅳ in rats’skeletal muscle tissues.Result After modeling,compared with the normal group,rats in the model,Zusanli,and non-acupoint groups showed emaciated,with decreased body weight and declined grip force(P<0.05);the activities of mitochondrial respiratory chain complexes Ⅰ,Ⅱ,Ⅲ,and Ⅳ and the content of ATP in skeletal muscle tissues dropped significantly in the three groups(P<0.05).Compared with the model group,rats in the Zusanli group had increased body weight and notably strengthened grip force(P<0.05),and the activities of mitochondrial respiratory chain complexes Ⅰ,Ⅱ,Ⅲ,and Ⅳ and the content of ATP in skeletal muscle tissues increased significantly(P<0.05);the differences between the non-acupoint group and model group were statistically insignificant in comparing the indicators mentioned above(P>0.05).Conclusion Electroacupuncture at Zusanli can significantly increase the activities of mitochondrial respiratory chain complexes Ⅰ,Ⅱ,Ⅲ,and Ⅳ and the content of ATP in skeletal muscles of spleen-Qi-deficie

关 键 词：电针 穴 足三里 脾气虚 线粒体呼吸链复合物 三磷酸腺苷 大鼠 

分 类 号：R2-03[医药卫生—中医学]