杨木心材白腐菌预处理及生物化机浆制浆性能研究  

Pretreatment of Poplar Heartwood by White-rot Fungus and Performance of Biological Chemi-mechanical Pulping

作　　者：罗文琪 谢益民[1,2] 张功侠 LUO Wenqi;XIE Yimin;ZHANG Gongxia(Research Institute of Pulp&Paper Engineering,Hubei University of Technology,Wuhan 430068,China;Hubei Provincial Key Laboratory of Green Light Industrial Materials,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学制浆造纸研究院,湖北武汉430068 [2]湖北工业大学绿色轻工材料湖北省重点实验室,湖北武汉430068

出　　处：《林产化学与工业》2022年第6期91-98,共8页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(21878070);湖北省高等学校优秀中青年科技创新团队计划资助项目(T201205)。

摘　　要：利用白腐菌Trametes sp.48424预处理杨木心材,然后进行生物化学机械法制浆,测定了白腐菌预处理杨木心材木片时的漆酶酶活,并分析了预处理前后杨木心材的化学组分的变化。利用扫描电镜(SEM)、红外光谱(FT-IR)、核磁共振碳谱(^(13)C NMR)等深入研究了白腐菌对杨木心材的降解机理,同时利用化学机械法制浆,探讨浆料纤维形态和成纸性能变化。研究结果表明:白腐菌能很好地在杨木心材木片上进行固态发酵,并在预处理第8天时漆酶酶活达到最大值1.3 IU/mL。在预处理阶段,白腐菌会使木片表面出现孔洞和凹槽,并且对Klason木质素的降解速率高于对综纤维素的降解速率。白腐菌预处理对磨木木质素(MWL)中的β-O-4结构的侧链(δ60.6和72.7)的降解较为明显,而对芳香环的影响较小,反映了白腐菌对木质素的降解主要发生在木质素侧链。与对照组相比,杨木心材木片经过白腐菌预处理后,纸浆的纤维长度、扭结指数增加,细小纤维含量减少,成纸强度显著提高。The white-rot fungus Trametes sp.48424 was used to pretreat poplar heartwood for the biochemical-mechanical pulp(Bio-CMP).The laccase activity of white-rot fungus during pretreatment was measured,and the changes in the chemical composition of poplar heartwood before and after pretreatment were also analyzed.Moreover,scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FT-IR),carbon-13 nuclear magnetic resonance(^(13)C NMR)were applied to intensively study the degradation mechanism of peplar heartwood by white-rot-fungus.Using Bio-CMP process,the changes of pulp fiber morphology and paper properties were discussed.The results showed that the white-rot fungus could perform solid-state fermentation well on the surface of the heartwood chips,and the laccase activity reached the maximum value of 1.3 IU/mL after 8 day of pretreatment.In the pretreatment stage,the white-rot fungus could lead to a porous and wrinkled surface structrue of the wood chip,and the degradation rate of Klason lignin was higher than that of holocellulose.White-rot fungus pretreatment could cleave β-O-4 side chain(δ60.6,72.7)substructure in milled wood lignin(MWL)effectively,while it had little effect on aromatic rings,reflecting that the degradation of lignin by white-rot fungus mainly happened on the lignin side chain.Compared with reference experiment,after poplar heartwood chips being pretreated by white-rot fungus,the fiber length and kinking index of the pulp increased,the content of fines reduced,and the paper strength enhanced obviously.

关 键 词：白腐菌 杨木心材 磨木木质素 纸张强度 

分 类 号：TQ35[化学工程] TS743[轻工技术与工程—制浆造纸工程]