漂洗降低白及militarine含量的响应面法研究  

Application of Response Surface Methodology for Optimication of Reducing the Content of Militarine in Bletilla striata by Rinsing

作　　者：陈默然 于华忠[1] 刘磊磊[1] 唐克华[1] CHEN Moran;YU Huazhong;LIU Leilei;TANG Kehua(Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,China)

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000

出　　处：《林产化学与工业》2022年第6期117-122,共6页Chemistry and Industry of Forest Products

基　　金：中央引导地方科技发展专项资金项目(2017XF5032)。

摘　　要：为了建立简便有效降低白及苦味的加工方法,采用响应面法探索了热水漂洗白及鲜茎切片的最佳工艺。以1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯(militarine)的含量为考察指标,通过单因素试验考察了漂洗温度、漂洗时间、漂洗液pH值对白及中militarine含量的影响,并利用Box-Behnken响应面分析法对漂洗工艺进行了优化。最佳漂洗工艺参数为:漂洗温度95℃、漂洗时间50 min、漂洗液pH值6.5,此条件下平行实验测得militarine质量分数下降比例达63.55%,漂洗3次后的白及鲜茎切片militarine质量分数下降比例达83.10%。在最佳工艺条件下,白及鲜茎切片以热水漂洗处理1~3次,可显著降低militarine含量。In order to establish a simple and effective processing method to reduce the bitterness of Bletilla striata(Thunb.ex A.Murray)Rchb.f.,response surface methodology was used to explore the best optimum conditions of hot water rinsing B.striata fresh stem slices.Taking the content of 1,4-bis[4-(glucoseoxy)benzyl]-2-isobutyl malate(militarine)as the index the effects of hot water rinsing temperature,rinsing time and pH value on the militarine content in B.striata were investigated by a single factor experiment,and the rinsing was optimized by Box-Behnken response surface analysis.The optimum conditions were obtained as follows:rinsing temperature of 95℃,rinsing time of 50 min,hot water pH of 6.5.Under these optimized conditions,the mass fraction of militarine decreased by 63.55% in parallel experiments,and the mass fraction of militarine in the fresh stem slices of B.striata reduced 83.10% after three rinses.Rinsing B.striata fresh stem slices with hot water for 1-3 times could significantly reduce the content of militarine under optimum conditions.

关 键 词：白及 militarine 热水漂洗 响应面法 

分 类 号：TQ35[化学工程] R284.1[医药卫生—中药学]