核心群奶牛超数排卵技术影响因素的研究  

Study on the Influence Factors of Superovulation in Nucleus Herd for Dairy Cattle

作　　者：郝海生[1] 赵善江[1] 杜卫华[1] 赵学明[1] 庞云渭[1] 邹惠影 宋金辉[1] 朱化彬[1] HAO Hai-sheng;ZHAO Shan-jiang;DU Wei-hua;ZHAO Xue-ming;PANG Yun-wei;ZOU Hui-ying;SONG Jin-hui;ZHU Hua-bin(Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国牛业科学》2022年第5期17-21,共5页China Cattle Science

基　　金：国家现代农业产业技术体系项目(CARS36);中国农业科学院科技创新工程协同创新任务项目(CAAS-XTCX2016011-01-07)。

摘　　要：[目的]为进一步研究核心群奶牛超数排卵技术影响因素。[方法]试验使用3个FSH剂量和4个公牛精液对27头青年奶牛进行超数排卵。对供体牛胚胎生产情况和受体胚胎移植妊娠率进行统计。[结果]结果显示,供体2次超排获得7.8枚平均可用胚胎显著高于1次超排获得的5.7枚平均可用胚胎(P<0.05);260,280 mg FSH超排供体获得6.0和7.7枚平均可用胚胎显著高于300 mg FSH超排供体获得的4.6枚平均可用胚胎(P<0.05);供体使用4个公牛精液人工授精后获得的平均可用胚胎数和平均未受精卵数差异不显著(P>0.05);移植前接种疫苗的受体胚胎移植妊娠率显著降低(P<0.05)。[结论]核心群奶牛超排适宜FSH剂量为260~280 mg,使用高受精力公牛精液和对超排反应好的供体重复超排能够提高超排效率,受体移植前避免疫苗接种能够提高胚胎移植妊娠率。[Objective] To investigate the effect of different factors on superovulation in core herd for dairy cattle. [Method] 27 donors were superovulated with three doses of FSH and 4 bull semen, respectively. Fresh embryos were transferred in recipients. [Result] The results showed that the mean(±SEM) number of transferable embryos(7.8±0.95) in double superovulation was significantly higher than the number of transferable embryos(5.7±0.91) in first superovulation(P<0.05). The mean number of transferable embryos(6.0±1.18 and 7.7±0.90) in the 260 and 280 mg FSH groups were significantly higher than the number of transferable embryos(4.6±1.29) in the 300 mg FSH group(P<0.05). There was no significant difference on the mean number of transferable embryos among 4 bull semen(P<0.05). Pregnancy rate of fresh embryo was affected significantly by vaccination in recipients(P<0.05). [Conclusion] Core herd could be superovulated with lower total amount of FSH and using bull semen of high fertilization ability for dairy cattle. Repeat superovulation could increase the number of transferable embryos. Avoiding vaccination might increase the pregnancy rate of embryo transfer in recipients.

关 键 词：奶牛 超数排卵 FSH 精液 胚胎移植 

分 类 号：S823[农业科学—畜牧学]