电针联合rTMS对脊髓损作大鼠损伤脊髓NGF、NT-3蛋白表达的影响  

Effect of EA Combined with rTMS on Protein Expressions of NGF and NT-3 in Rats with SCI

作　　者：曹祖懋 赵亚莉 叶青 胡煜 李志刚[1] 时素华 姚海江[3] CAO Zumao;ZHAO Yali;YE Qing;HU Yu;LI Zhigang;SHI Suhua;YAO Haijiang(School of Acu-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 102488,China;The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China;Treatment Center of Traditional Chinese Medicine,Beijing Bo’ai Hospital,China Rehabilitation Research Center,Beijing 100068,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京102488 [2]北京中医药大学第三附属医院,北京100029 [3]中国康复研究中心北京博爱医院,北京100068

出　　处：《针灸临床杂志》2022年第12期71-77,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金青年项目,编号:82004485;北京中医药大学青年教师项目,编号:2019-JYB-JS-176。

摘　　要：目的:观察电针联合rTMS对脊髓损伤(SCI)大鼠损伤脊髓NGF、NT-3蛋白表达的影响。方法:SD大鼠120只随机分为空白组、假手术组、模型组、电针组、rTMS组与联合组(电针+rTMS),其中模型组、电针组、rTMS组和联合组再随机分为1 d组与7 d组,各12只。电针组给予电针治疗,rTMS组给予rTMS治疗,联合组则先进行rTMS治疗后再进行电针治疗。采用BBB评分观察治疗前后运动功能的改善程度,HE、尼氏染色观察神经元恢复情况,Westernblot检测NGF、NT-3蛋白表达的情况。结果:造模后模型和各干预组NGF、NT-3含量下降,治疗7 d后,各干预组与模型组比较NGF、NT-3增加(P<0.05或P<0.01),电针组与rTMS组比较NGF含量增加(P<0.01),联合组与rTMS组比较NGF含量增加(P<0.01),联合组与电针组比较NGF含量增加(P<0.05)。rTMS组与模型组比较NT-3含量增加,差异无统计学意义(P>0.05),电针组、联合组与模型组比较NT-3含量增加(P<0.01),电针组与rTMS组比较NT-3含量增加,差异无统计学意义(P>0.05),联合组与rTMS组比较NT-3含量,差异无统计学意义增加(P<0.01),联合组与电针组相比NT-3含量增加(P<0.05)。结论:电针联合rTMS能促进NGF、NT-3蛋白的表达,促进SCI的恢复。Objective:To observe the effect of electro-acupuncture(EA)combined with rTMS on the protein expressions of NGF and NT-3 in the rats with spinal cord injured(SCI).Methods:120 SD rats were randomly divided into the blank group,the sham operation group,the model group,the EA group,the rTMS group and the combined group(EA plus rTMS).The model group,the EA,the rTMS group and the combined group were then randomly divided into 1 d group and the 7 d group,with 12 rats in each group.The combined group was treated with rTMS first then followed by EA.BBB was used to observe the improvement of motor function before and after the treatment,HE and Nissl staining were used to observe the recovery of neurons,and Western blot was used to detect the protein expressions of NGF and NT-3.Results:The contents of NGF and NT-3 were decreased in the model group and in the intervention groups after modeling.After 7 days of treatment,the contents of NGF and NT-3 were increased in the intervention groups compared with those in the model group(P<0.05,P<0.01,P<0.01);the content of NGF was increased in the EA group compared with that in the rTMS group(P<0.01);the content of NGF in the combined group was increased compared with that in the rTMS group and in the EA group(P<0.01,P<0.05).Compared with that in the model group,the content of NT-3 was increased in the rTMS group,but there was no statistical difference between the two groups(P>0.05);the content of NT-3 was increased in the EA group and in the combined group compared with that in the model group(P<0.01,P<0.01);the content of NT-3 was increased in the EA group compared with that in the rTMS group,but there was no statistical difference between the two groups(P>0.05);the content of NT-3 was increased in the combined group compared with that in the rTMS group and in the EA group(P<0.01,P<0.05).Conclusion:EA combined with rTMS can increase the protein expressions of NGF and NT-3 to promote the recovery of SCI.

关 键 词：脊髓损伤 电针 RTMS 

分 类 号：R246.6[医药卫生—针灸推拿学]