栽培花生种皮内表皮颜色的遗传分析  

Genetic Analysis on Inner Epidermal Color of Peanut Seed Coat

作　　者：王菲菲 张胜忠 胡晓辉[1] 苗华荣[1] 于豪谅 陈静[1] WANG Fei-fei;ZHANG Sheng-zhong;HU Xiao-hui;MIAO Hua-rong;YU Hao-liang;CHEN Jing(Shandong Peanut Research Institute,Qingdao 266100,China;Yantai Fenglin Foodstuff Co.,Ltd,Yantai 264108,China)

机构地区：[1]山东省花生研究所,山东青岛266100 [2]烟台枫林食品股份有限公司,山东烟台264108

出　　处：《花生学报》2022年第4期8-17,共10页Journal of Peanut Science

基　　金：国家自然科学基金项目(32001584);山东省农业良种工程(2020LZGC001);山东省农业科学院创新工程(CXGC20A03,CXGC20A21);青岛市民生科技计划项目(20-3-4-26-nsh)。

摘　　要：本研究以花育36号×F18构建重组自交系(recombinant inbred line,RIL)群体为试验材料,考察2个环境下(E1、E2)RIL群体种皮内表皮颜色RGB值表型数据,采用数量性状主基因+多基因混合遗传分析R软件包SEAv2.0进行遗传分析。结果表明:花生种皮内表皮颜色R值的最优遗传模型是MX2-AE-A,即2对加性-上位性-加性的主基因+多基因遗传模型;第一对主基因的加性效应值(da)为-6.14,第一个主基因加性×第二个主基因加性上位性互作效应值为-0.87,主基因遗传率为79.28%。花生种皮内表皮颜色G值的最优遗传模型为PG-AI,即加性-上位性的多基因遗传模型;主基因遗传率为0,多基因遗传率为99.98%,花生种皮内表皮颜色R值主要由多基因控制。花生种皮内表皮颜色B值最优遗传模型是MX3-AI-A,即由3对加性-上位性-加性主基因+多基因遗传模型;主基因间的整体上位性互作效应(i*)远小于其整体加性效应(d*),主基因遗传率为97.60%。本研究为深入开展花生种皮内表皮颜色分子机制研究奠定了重要基础。A recombinant inbred line(RIL)population derived from a cross between Huayu36 and F18 was constructed,and traits of R value,G value and B value of the RIL population were investigated under two environments(E1,E2).And SEA v2.0,an R software package for mixed major genes plus polygenes inheritance analysis of quantitative traits,was used to analyze the phenotypic data.The optimal genetic model for R value was MX2-AE-A,i.e.,two additive-epistatic-additive major+polygenes model.The addictive effect of the first pair major gene was-6.14,additive effect plus additive effect of the 1 st pair major gene×the 2 nd pair major gene was-0.87,and the genetic efficiency of the main genes was 79.28%.The optimal genetic model for G value was PG-AI,i.e.,additive-epistatic polygenes model,the genetic efficiency of the main genes was 0 and genetic efficiency of the polygenes was 99.98%,indicating that the inner epidermal color G value of peanut seed coat was mainly controlled by polygenes.The optimal genetic model for B value was MX3-AI-A,i.e.,three additive-epistatic-additive major+polygenes model.The additive×additive interaction of(i*)between major genes was far less than additive effect of the major gene(d*),the genetic efficiency of the main genes was 97.60%.This work provided a valuable foundation for further study on the molecular mechanism regulating the inner epidermal color of peanut seed coat.

关 键 词：花生 种皮内表皮颜色 RGB值 主基因+多基因 遗传分析 

分 类 号：Q348[生物学—遗传学] S565.203.2[农业科学—作物学]