16份花生品种(系)油酸含量及FAD2基因型鉴定  

The Oleic Content and FAD2 Genotype Identification of 16 Peanut Cultivars(Lines)

作　　者：王菲菲 张胜忠 胡晓辉[1] 苗华荣[1] 许林英 吴兰荣 李春娟[1] 陈静[1] WANG Fei-fei;ZHANG Sheng-zhong;HU Xiao-hui;MIAO Hua-rong;XU Lin-ying;WU Lan-rong;LI Chun-juan;CHEN Jing(Shandong Peanut Research Institute,Qingdao 266100,China;Cixi Agricultural Technology Extension Center,Cixi 315300,China;Qingdao Agricultural Technology Extension Center,Qingdao 266071,China)

机构地区：[1]山东省花生研究所,山东青岛266100 [2]慈溪市农业技术推广中心,浙江慈溪315300 [3]青岛市农业技术推广中心,山东青岛266071

出　　处：《花生学报》2022年第4期29-34,43,共7页Journal of Peanut Science

基　　金：新疆维吾尔自治区重大科技专项(2022A02008-3);国家自然科学基金项目(32001584);山东省农业良种工程项目(2020LZGC001);山东省农业科学院创新工程项目(CXGC20A03,CXGC20A21);青岛市民生科技计划项目(20-3-4-26-nsh)。

摘　　要：为研究花生油酸含量与AhFAD2基因型的关系,选用低(小于50%)、中(50%~70%)、高油酸(大于75%)含量的16个品种(系)进行分析。通过竞争性等位基因PCR(kompetitive allele specific PCR,KASP)和直接测序法对AhFAD2进行分型,结果表明低油酸品种(系)的基因型均为AABB,中油酸品种(系)的基因型为aaBB,高油酸品种(系)的基因型为aabb。7个高油酸品种(系)的AhFAD2B突变为F435突变,即442位A碱基插入,而06B16的AhFAD2B突变为665位205 bp MITE转座子的插入。对16个花生品种(系)控制油酸基因型的鉴定,尤其是高油酸种质06B16基因型的确定将为高油酸花生分子育种提供理论参考。In order to study the relationship between oleic acid content and AhFAD2 genotype,16 cultivars(lines)with low(less than 50%),medium(50%~70%),and high(more than 75%)oleic acid content were selected for analysis.AhFAD2 was genotyped by KASP(kompetitive allele specific PCR)and direct sequencing.The results showed that the genotype of low oleate cultivars(lines)were AABB,of medium oleic acid cultivars(lines)were aaBB,and of high oleate cultivars(lines)were all aabb.It was an insertion of 442 A on AhFAD2B in the seven high oleate cultivars(lines),however,it was an insertion of MITE(miniature inverted-repeat transposable element)at position 665 on AhFAD2B.The identification of AhFAD2 genotype in 16 peanut varieties(lines),especially the genotype of high oleate 06 B16,will provide theoretical reference for molecular breeding of high oleate peanut varieties(lines).

关 键 词：花生 油酸含量 AhFAD2 基因型 

分 类 号：S565.203.3[农业科学—作物学] Q754[生物学—分子生物学]