六项联合指标在阴道微生态评价中的应用价值  

Application value of six joint indicators in vaginal microecology evaluation

作　　者：周小微 ZHOU Xiaowei(Department of Laboratory Medicine,the Nanping First Affiliated Hospital of Fujian Medical University,Fujian,Nanping 353000,China)

机构地区：[1]福建医科大学附属南平第一医院检验科,福建南平353000

出　　处：《中国医药科学》2022年第24期193-196,共4页China Medicine And Pharmacy

摘　　要：目的 研究细菌性阴道炎(BV)、需氧菌性阴道炎(AV)六项联合检测在阴道微生态评价中的价值。方法选取2021年1—5月在福建医科大学附属南平第一医院妇产科门诊进行检查的患者2960例,对受检患者的阴道分泌物进行常规湿片镜检、革兰氏染色涂片镜检及采用阴道炎六项联合检测进行测定。比较两种检测方法的最终结果及菌群异常、正常预成酶阳性率。结果 菌群正常1200例(40.54%)、菌群抑制239例(8.07%)、菌群失调1112例(37.57%)、菌群过度增殖59例(1.99%)、病原体被检出350例(11.83%)。BV经两种方法检测结果的符合率为94.76%,阴道炎六项联合检测与革兰氏染色涂片镜检BV阳性率比较,差异无统计学意义(P>0.05)。阴道炎六项联合检测与革兰氏染色涂片镜检AV阳性率,差异有统计学意义(P<0.05)。菌群正常预成酶阳性率低于菌群异常,差异有统计学意义(P<0.05),但其中凝固酶阳性率比较,差异无统计学意义(P>0.05)。结论 阴道炎六项联合检测对BV感染的诊断具有较高的可靠性,但单独应用于AV感染的判定尚存在一定局限性,通过该方法显示应以形态学检测为主、功能学为辅对阴道微生态情况进行准确的评估。Objective To study the value of six joint tests for bacterial vaginosis(BV) and aerobic vaginitis(AV) detection in the vaginal microecology evaluation. Methods A total of 2960 women visiting the outpatient clinics of the Department of Obstetrics and Gynecology of The Nanping First Affiliated Hospital of Fujian Medical University from January to May 2021 were selected. The vaginal secretions of the selected women were examined by conventional wet mount microscopy, Gram-stained smear microscopy versus six joint tests for AV and BV detection. The final results, flora abnormality and positive rate of normal preformation zymogram obtained by the two methods were compared. Results Six joint tests for vaginitis among 2960 patients showed: 1200 cases(40.54%) with normal flora, 239 cases(8.07%) with inhibition of flora, 1112 cases(37.57%) with dysbacteriosis,59 cases(1.99%) with excessive proliferation of flora, and 350 cases(11.83%) with pathogens detected. The coincidence rate of BV detected by the two methods was 94.76%. There was no significant difference in the positive rate of BV between the combined detection of six items of vaginitis and Gram staining smear microscopy(P > 0.05). There was a statistically significant difference in the positive rate of AV between the six combined tests of vaginitis and Gram staining smear microscopy(P< 0.05). The positive rate of normal preformase of the flora was lower than that of abnormal flora, the difference was statistically significant(P< 0.05), but the positive rate of coagulase was not statistically significant(P > 0.05). Conclusion The six joint tests for vaginitis show a high reliability in the diagnosis of BV infection, but there are still some limitations when they are used alone to determine AV infection. During the application of this method, the morphology detection should be the focus,supplemented by the functional detection to accurately evaluate the vaginal microecology.

关 键 词：细菌性阴道病 需氧菌性阴道炎 阴道炎六项联合检测 革兰氏染色涂片 

分 类 号：R711.3[医药卫生—妇产科学]