呼吸道合胞病毒肺炎患儿外周血单个核细胞IFITM3、IFI27表达变化及其临床意义  

Expression changes of IFITM3 and IFI27 in peripheral blood mononuclear cells of children with respiratory syncytial virus pneumonia and their clinical significance

作　　者：薛亚琪 沙宁 武怡 XUE Yaqi;SHA Ning;WU Yi(Department of Pediatrics,Huai'an Hospital Affiliated to Xuzhou Medical University,Huai'an Second People's Hospital,Huai'an 223002,China;不详)

机构地区：[1]徐州医科大学附属淮安医院淮安市第二人民医院儿科,江苏淮安223002 [2]徐州医科大学附属医院儿科

出　　处：《山东医药》2023年第1期19-23,共5页Shandong Medical Journal

基　　金：江苏省妇幼健康科研项目(F202151);淮安市自然科学研究计划(HAB202114)。

摘　　要：目的探讨呼吸道合胞病毒(RSV)肺炎患儿外周血单个核细胞干扰素诱导跨膜蛋白3(IFITM3)、干扰素诱导蛋白27(IFI27)表达变化及其临床意义。方法选择RSV肺炎患儿90例(观察组),其中病情程度低危28例、中危30例、高危32例,选择同期腹股沟斜疝患儿40例(对照组),采集所有研究对象外周静脉血,采用荧光定量PCR法检测外周血单个核细胞IFITM3、IFI27表达,采用ELISA法检测血清IL-1β、IL-6、TNF-α、IFN-γ,采用肺功能检测仪检测第1秒用力呼气容积占预计值的百分比(FEV1%pred)、FEV1/用力肺活量(FVC)。采用Pearson相关分析法分析RSV肺炎患儿外周血单个核细胞IFITM3、IFI27表达与血清IL-1β、IL-6、TNF-α、IFN-γ水平及FEV1%pred、FEV1/FVC的关系。采用受试者工作特征(ROC)曲线分析外周血单个核细胞IFITM3、IFI27表达对RSV肺炎的诊断价值。结果观察组外周血单个核细胞IFITM3、IFI27相对表达量及血清IL-1β、IL-6、TNF-α、IFN-γ水平均显著高于对照组,FEV1%pred、FEV1/FVC均显著低于对照组(P均<0.01)。随着病情程度加重,RSV肺炎患儿外周血单个核细胞IFITM3、IFI27相对表达量及血清IL-1β、IL-6、TNF-α、IFN-γ水平逐渐升高,FEV1%pred、FEV1/FVC逐渐降低(P均<0.01)。RSV肺炎患儿外周血单个核细胞IFITM3、IFI27表达与血清IL-1β、IL-6、TNF-α、IFN-γ水平均呈正相关关系,与FEV1%pred、FEV1/FVC均呈负相关关系(P均<0.05)。ROC曲线分析显示,外周血单个核细胞IFITM3、IFI27表达单独和联合预测RSV肺炎的曲线下面积(AUC)分别为0.756、0.763、0.837,外周血单个核细胞IFITM3、IFI27表达联合预测RSV肺炎的AUC高于外周血单个核细胞IFITM3、IFI27表达单独(Z分别为2.096、2.115,P均<0.05)。结论RSV肺炎患儿外周血单个核细胞IFITM3、IFI27表达显著升高,二者表达与血清IL-1β、IL-6、TNF-α、IFN-γ水平均呈正相关关系;外周血单个核细胞IFITM3、IFI27表达对RSV肺炎均有一定预测价值,Objective To investigate the expression changes and clinical significance of interferon-induced transmembrane protein 3(IFITM3)and interferon alpha-inducible protein 27(IFI27)in peripheral blood mononuclear cells of children with respiratory syncytial virus(RSV)pneumonia.Methods Ninety children with RSV pneumonia(observation group)were selected,including 28 low-risk cases,30 medium-risk cases,and 32 high-risk cases.Forty children with indirect inguinal hernia(control group)were selected at the same time.Peripheral venous blood was collected to detect IFITM3 and IFI27 expression levels of peripheral blood mononuclear cells by fluorescent quantitative PCR,and serum levels of IL-1β,IL-6,TNF-α,and IFN-γ were detected by ELISA.The day after admission,the percentage of forced expiratory volume in 1 second to the predicted value(FEV1%pred)and FEV1/forced vital capacity(FVC)were measured by pulmonary function detector.Pearson correlation analysis was used to analyze the relationships between the expression levels of IFITM3 and IFI27 in peripheral blood mononuclear cells and serum levels of IL-1β,IL-6,TNF-α,IFN-γ,FEV1%pred and FEV1/FVC in children with RSV pneumonia.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of IFITM3 and IFI27 expression in peripheral blood mononuclear cells for RSV pneumonia.Results The relative expression levels of IFITM3 and IFI27 in the peripheral blood mononuclear cells and the serum levels of IL-1β,IL-6,TNF-α,and IFN-γ in the observation group were significantly higher than those in the control group,and the levels of FEV1%pred and FEV1/FVC were significantly lower than those in the control group(all P<0.01).With the severity of RSV pneumonia,the relative expression levels of IFITM3 and IFI27 in peripheral blood mononuclear cells and the serum levels of IL-1β,IL-6,TNF-α and IFN-γ gradually increased,while the levels of FEV1%pred and FEV1/FVC gradually decreased(all P<0.01).The expression levels of IFITM3 and IFI27 in peripheral blood mononuclear

关 键 词：呼吸道合胞病毒肺炎 干扰素诱导跨膜蛋白3 干扰素诱导蛋白27 儿童 

分 类 号：R725.6[医药卫生—儿科]