梅花鹿鹿茸总蛋白对糖尿病肾病大鼠肾损伤的治疗作用及分子机制  

Therapeutic effect and molecular mechanism of Sika deer velvet antler protein on renal injury of rats with diabetes nephropathy

作　　者：崔艳[1] 赵晓露 师晶晶 刘云[1] CUI Yan;ZHAO Xiaolu;SHI Jingjing;LIU Yun(Hospital of Nephrology,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China)

机构地区：[1]新乡医学院第一附属医院肾脏病医院,河南卫辉453100

出　　处：《新乡医学院学报》2023年第1期11-17,共7页Journal of Xinxiang Medical University

基　　金：河南省医学科技攻关计划联合共建项目(编号:LHGJ20210511)。

摘　　要：目的探讨梅花鹿鹿茸总蛋白提取物(SVPr)对糖尿病肾病(DN)大鼠肾损伤的治疗作用及分子机制。方法将120只Sprague Dawley大鼠按照简单随机数字表法分为对照组、模型组、二甲双胍组、低剂量SVPr组、中剂量SVPr组、高剂量SVPr组,每组20只。模型组、二甲双胍组、低剂量SVPr组、中剂量SVPr组、高剂量SVPr组大鼠造模前禁食12 h,单次腹腔注射链脲佐菌素50 mg•kg^(-1)制备DN大鼠模型;造模成功后,二甲双胍组大鼠给予二甲双胍500 mg•kg^(-1)•d^(-1)灌胃,低剂量SVPr组、中剂量SVPr组、高剂量SVPr组大鼠分别经尾静脉注射质量浓度为1.47、2.94、4.41 g•L^(-1)的SVPr溶液0.1 mL,对照组及模型组大鼠注射等量生理盐水,每日1次,持续给药4周。采用生物化学法检测各组大鼠血尿素氮(BUN)、血肌酐(SCr)、血糖、24 h尿蛋白水平,酶联免疫吸附试验法检测各组大鼠肾组织中活性氧(ROS)、核因子-κB(NF-κB)水平,Western blot法检测各组大鼠肾组织中沉默调节蛋白1(SIRT1)、核因子E2相关因子2(Nrf-2)、血红素加氧酶-1(HO-1)蛋白相对表达量。结果模型组大鼠BUN、SCr、24 h尿蛋白、血糖水平均显著高于对照组(P<0.05);低剂量SVPr组、中剂量SVPr组、高剂量SVPr组和二甲双胍组大鼠BUN、SCr、24 h尿蛋白、血糖水平显著低于模型组(P<0.05);高剂量SVPr组大鼠SCr、24 h尿蛋白水平显著低于二甲双胍组(P<0.05),高剂量SVPr组与二甲双胍组大鼠BUN、血糖水平比较差异无统计学意义(P>0.05);中剂量SVPr组大鼠SCr水平显著低于二甲双胍组,BUN、血糖水平显著高于二甲双胍组(P<0.05);中剂量SVPr组与二甲双胍组大鼠24 h尿蛋白水平比较差异无统计学意义(P>0.05);低剂量SVPr组大鼠BUN、24 h尿蛋白、血糖水平显著高于二甲双胍组(P<0.05),低剂量SVPr组与二甲双胍组大鼠SCr水平比较差异无统计学意义(P>0.05)。高剂量SVPr组大鼠BUN、血糖、24 h尿蛋白、SCr水平显著�Objective To investigate the therapeutic effect and molecular mechanism of Sika deer velvet antler protein(SVPr)on renal injury in diabetes nephropathy(DN)rats.Methods A total of 120 Sprague Dawley rats were divided into control group,model group,metformin group,low-dose of SVPr group,medium-dose of SVPr group and high-dose of SVPr group according to the simple random number table,with 20 rats in each group.The rats in the model group,metformin group,low-dose of SVPr group,medium-dose of SVPr group and high-dose of SVPr group were fasted for 12 hours before modeling,and the rats were single intraperitoneally injected with streptozocin 50 mg•kg^(-1) to prepare the DN model.After successful modeling,the rats in the metformin group were given metformin by gavage with a dose of 500 mg•kg^(-1)•d^(-1);the rats in the low-dose of SVPr group,medium-dose of SVPr group and high-dose of SVPr group were injected via tail vein with 0.1 mL SVPr solution with concentrations of 1.47,2.94 and 4.41 g•L^(-1),respectively;the rats in the control group and model group were injected with the same amount of normal saline,once a day for 4 weeks.The levels of blood urea nitrogen(BUN),serum creatinine(SCr),blood glucose and 24 h urine protein of rats in each group were detected by biochemical method,and the levels of reactive oxygen species(ROS)and nuclear factor-κB(NF-κB)in kidney tissue of rats in each group were detected by enzyme-linked immunosorbent assay,the relative expressions of silencing regulatory protein 1(SIRT1),nuclear factor erythroid-2 related factor 2(Nrf-2),heme oxygenase-1(HO-1)protein in renal tissues of rats in each group were detected by Western blot.Results The levels of BUN,SCr,24 h urine protein and blood glucose of rats in the model group were significantly higher than those in the control group(P<0.05);the levels of BUN,SCr,24 h urine protein and blood glucose of rats in the low-dose of SVPr group,medium-dose of SVPr group,high-dose of SVPr group and metformin group were significantly lower than those

关 键 词：梅花鹿鹿茸总蛋白 糖尿病肾病 沉默调节蛋白1 核因子E2相关因子2 血红素加氧酶-1 活性氧 核因子-ΚB 

分 类 号：R587.2[医药卫生—内分泌]