不同剂量彝药小红参乙酸乙酯提取物预防灌胃给药对大鼠心肌缺血再灌注损伤的防治作用及其机制  

Preventive and therapeutic effect of different doses of ethyl acetate extracts of Yi medicine Rubia yunnanensis(Franch.)Diels on rats with myocardial ischemia-reperfusion injury

作　　者：肖湉 王礴 杨丽萍 段小花 XIAO Tian;WANG Bo;YANG Liping;DUAN Xiaohua(School of Ethnic Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学民族医药学院云南省傣医药与彝医药重点实验室,昆明650500

出　　处：《山东医药》2022年第36期38-43,共6页Shandong Medical Journal

基　　金：云南省科技厅—云南中医学院应用基础研究联合专项[2018FF001-076];兴滇英才支持计划—青年人才专项(2022)。

摘　　要：目的 观察不同剂量彝药小红参乙酸乙酯提取物预防灌胃给药对大鼠心肌缺血再灌注损伤(MIRI)的防治作用,并探讨其作用机制。方法 同一批次二导联心电图检测合格的大鼠60只,随机分为6组各10只。小红参高剂量组、小红参中剂量组、小红参低剂量组制模前分别灌胃给予0.028、0.017、0.005 67 g/mL的小红参乙酸乙酯提取物,每日一次饲养灌胃7 d后进行MIRI操作;复方丹参组制模前灌胃给予复方丹参片水磨液1 mL/(100 g·d),饲养灌胃7 d后进行MIRI操作;MIRI组灌胃给予同体积1 mL/(100 g·d)蒸馏水,饲养灌胃7 d后进行MIRI操作;假手术组灌胃给予同体积1 mL/(100 g·d)蒸馏水,饲养灌胃7 d后不进行MIRI操作,仅穿线但不结扎。比较各组大鼠心电图、心肌病理组织学、心肌梗死面积及血清心肌肌钙蛋白I(cTnI)、肌酸激酶同工酶(CK-MB)、谷胱甘肽过氧化物酶(GSH-PX)、总超氧化物歧化酶(SOD)、丙二醛(MDA)和心肌组织Nrf2、超氧化物歧化酶1(SOD1)、血红素氧合酶1(HO-1)、醌氧化还原酶1(NQO1)mRNA。结果 与MIRI组比较,小红参高剂量组、小红参中剂量组、小红参低剂量组在再灌注后ST段明显回落,接近正常范围,心肌梗死面积减少,CK-MB、cTnI和MDA含量降低,SOD、GSH-Px含量和NQO1、SOD1、Nrf2、HO-1 mRNA表达升高,尤以小红参高剂量组最为明显(P均<0.05)。结论 不同剂量小红参乙酸乙酯提取物预防灌胃给药均对大鼠心肌缺血再灌注损伤有防治作用,尤以0.028 g/mL效果最佳,机制可能与其激活氧化信号通路Nrf2/ARE从而减轻氧化应激损伤有关。Objective To observe the preventive and therapeutic effect of intragastric administration of different dos-es of ethyl acetate extracts of Yi medicine Rubia yunnanensis(Franch.)Diels(Rubia yunnanensis)on rats with myocardi-al ischemia-reperfusion injury(MIRI),and to explore its mechanism.Methods Sixty rats with the same batch of twolead ECG were selected and randomly divided into six groups,with 10 rats in each. Rats in the high-dose,medium-dose,and low-dose Rubia yunnanensis groups were given 0. 028,0. 017,0. 005 67 g/m L of ethyl acetate extracts of Rubia yun-nanensis intragastrically,once a day;after 7 days,MIRI operations were prepared. Rats in the compound Danshen groupwere given 1 m L/(100 g·d)of water mill solution before modeling,and the MIRI operation was prepared after feeding for7 days. Rats in the MIRI group were given the same volume of 1 m L/(100 g·d)of distilled water for gavage,and the MIRIoperation was prepared after feeding for 7 days. Rats in the sham group were given 1 m L/(100 g·d)of distilled water forgavage,and after 7 days,the rats were threaded but were not ligated. The electrocardiogram,myocardial pathological his-tology,myocardial infarction area,serum cardiac troponin I(c Tn I),creatine kinase isoenzyme(CK-MB),glutathioneperoxidase(GSH-PX),total superoxide dismutase(SOD),malondialdehyde(MDA),myocardial tissue Nrf2,superoxidedismutase 1(SOD1),heme oxygenase 1(HO-1),and quinone oxidoreductase 1(NQO1)m RNA of rats were compared.Results Compared with the MIRI group,the ST segment decreased significantly after reperfusion,which was close to the normal range,the myocardial infarction area decreased,the content of CK-MB,c Tn I and MDA decreased,and the expres-sion levels of SOD,GSH-Px and NQO1,SOD1,Nrf2 and HO-1 m RNA increased in the high-dose,medium-dose,andlow-dose Rubia yunnanensis groups,especially in the high-dose Rubia yunnanensis group(all P<0. 05).Conclusion Different doses of ethyl acetate extracts of Rubia yunnanensis have preventive and therapeutic effect on MIRI rats,especial-

关 键 词：心肌缺血再灌注损伤 小红参 氧化应激 Nrf2/ARE通路 

分 类 号：R542.2[医药卫生—心血管疾病]