甘肃省野生岩羊不明原因死亡分析  

Analysis of unexplained deaths of wild Pseudois nayaur in Gansu province,China

作　　者：张丹 色拥军 付国明 贺兆锴 吕东月 春花 段然[1] 秦帅 梁俊容[1] 肖萌 景怀琦[1] 王鑫[1] ZHANG Dan;SE Yong-jun;FU Guo-ming;HE Zhao-kai;LYU Dong-yue;CHUN Hua;DUAN Ran;QING Shuai;LIANG Jun-rong;XIAO Meng;JING Huai-qi;WANG Xin(Emergency Response Laboratory,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Yanchiwan National Nature Reserve Administration of Subei,Subei,Gansu 736300,China;Subei Mongolian Autonomous County Center for Disease Control and Prevention,Subei,Gansu 736300,China;Hangzhou Center for Disease Control and Prevention,Hangzhou,Zhejiang 310021,China;Pudong New Area Center for Disease Control and Prevention,Shanghai 200120,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所应急实验室,北京102206 [2]甘肃盐池湾国家级自然保护区管理局,甘肃肃北736300 [3]甘肃省肃北蒙古族自治县疾病预防控制中心,甘肃肃北736300 [4]杭州市疾病预防控制中心,浙江杭州310021 [5]浦东新区疾病预防控制中心,上海200120

出　　处：《中国媒介生物学及控制杂志》2022年第6期888-894,共7页Chinese Journal of Vector Biology and Control

基　　金：国家科技重大专项(2018ZX10713-001-002,2018ZX10713-003-002)。

摘　　要：目的 探明甘肃省野生岩羊异常死亡的原因。方法 运用分子克隆的方法,挑选多个样本核酸16S rRNA基因克隆子,通过测序技术得到基因序列,在美国国立生物技术信息中心(NCBI)数据库中用BLAST进行比对,并结合鞭毛蛋白基因多重PCR检测,进行死因探索。结果 2018年12月-2020年4月发现35只岩羊异常死亡,在4只岩羊的5个组织标本核酸中检测到致病梭状芽孢杆菌属细菌的16S rRNA基因序列,其中2只岩羊的3个标本核酸均是致病梭状芽孢杆菌属细菌丰度最高(61.90%~91.12%);其他标本核酸16S rRNA基因克隆子中,均是非梭状芽孢杆菌属细菌丰度最高,其中5只岩羊丰度最高的为反刍动物正常肠道细菌。结论 多种梭状芽孢杆菌属细菌感染和消化道正常细菌的易位,可能分别是野生岩羊的主要致死原因和次要致死原因。Objective To investigate the reasons for the abnormal deaths of wild Pseudois nayaur in Gansu province,China. Methods By molecular cloning, 16S rRNA gene clones were selected from the nucleic acid of multiple specimens. The gene sequences were obtained by sequencing technology and compared with the sequences in National Center for Biotechnology Information database by the basic local alignment search tool. By combining with the multiplex polymerase chain reaction(PCR) for flagellin gene, the causes of the deaths were analyzed. Results Total 35abnormally died wild P. nayaur were found from December 2018 to April 2020. The 16S rRNA gene sequences of pathogenic Clostridium species were found in the nucleic acid of five tissue specimens from four P. nayaur. The abundance of pathogenic Clostridium spp. was the highest in the nucleic acid of three specimens from two P. nayaur(61.90%-91.12%). Among the 16S rRNA gene clones in the nucleic acid of the other specimens, the abundance of nonClostridium spp. was the highest, and the abundance of normal intestinal bacteria of ruminant was the highest in the specimens from five P. nayaur. Conclusion The multiple infection of Clostridium spp. might be the main cause of death of these wild P. nayaur in this study, and translocation of normal intestinal bacteria might be the secondary cause of death of these wild P. nayaur.

关 键 词：岩羊 诺维梭状芽孢杆菌 产气荚膜梭菌 索氏梭状芽孢杆菌 16S rRNA基因 鞭毛蛋白基因 

分 类 号：R378.2[医药卫生—病原生物学]