基于靶向代谢组学的冠心病阵发性心房颤动痰浊证患者血清胆汁酸谱研究  

Study on serum bile acid profile of coronary heart disease combined with paroxysmal atrial fibrillation patients with stagnant phlegm syndrome based on targeted metabolomics

作　　者：褚瑜光[1] 柴若宁 杨亦含 袁果真 CHU Yu-guang;CHAI Ruo-ning;YANG Yi-Han;YUAN Guo-zhen(Department of Cardiovascular Diseases,Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院心血管科,北京100053

出　　处：《北京中医药》2022年第10期1102-1109,共8页Beijing Journal of Traditional Chinese Medicine

基　　金：中央级公益性科研院所基本科研业务费专项(ZZ15-XY-LCQ-02);首都卫生发展科研专项(首发2022-1-4153);中国中医科学院科技创新工程项目(CI2021A00918)。

摘　　要：目的寻找冠心病阵发性心房颤动(CHD-PAF)心脾阳虚、痰浊内阻证(简称痰浊证)血清胆汁酸相关的潜在生物标志物。方法利用超高效液相色谱一三重四极杆质谱联用仪(UHPLC-MS/MS)技术检测CHD-PAF痰浊证和非痰浊证患者以及健康人的43种血清胆汁酸的浓度和含量,使用偏最小二乘方判别分析(PLS-DA)比较3组受试者的血清胆汁酸组代谢轮廓,使用相关性分析方法分析血清胆汁酸与CHD-PAF痰浊证患者的临床表型及证候发生的相关性,筛选CHDPAF痰浊证的潜在胆汁酸生物标记物。结果CHD-PAF痰浊证患者的血清胆汁酸谱较非痰浊证患者发生显著变化,血清胆汁酸水平较非痰浊证患者显著升高,并与CHD-PAF痰浊证的发生存在相关性,其中以总胆汁酸、次级胆汁酸和游离胆汁酸的相关性最强。结论血清总胆汁酸、次级胆汁酸和游离胆汁酸升高可作为CHD-PAF心脾阳虚、痰浊内阻证临床辨证的潜在生物标记物。Objective To explore the potential biomarkers related to serum bile acids for coronary heart disease combined with paroxysmal atrial fibrillation(CHD-PAF)patients with heart-spleen yang deficiency and stagnant phlegm syndrome.Methods The concentration and contents of 43 kinds of serum bile acids(BAs)in CHD-PAF patients with stagnant phlegm syndrome and non-stagnant phlegm syndrome and healthy population were detected by ultra-high performance liquid chromatography-mass spectrometry.Partial least square discriminant analysis(PLS-DA)was used to compare the metabolic profile of serum BAs among three test groups.Correlation analysis was used to analyze the correlation between serum BAs and the clinical phenotype,as well as BAs and occurrence of stagnant phlegm syndrome,and to screen the potential biomarkers related to serum BAs for CHD-PAF patients with stagnant phlegm syndrome.Results Compared with CHD-PAF patients with non-stagnant phlegm syndrome,the serum bile acid level was significantly higher than that of patients with non-stagnant phlegm syndrome,and it was correlated with the occurrence of CHD-PAF phlegm syndrome.With the strongest correlations being found for total,secondary and unconjugated BAs in particular.Conclusion Elevated serum total BAs,secondary BAs and unconjugated BAs can be used as potential biomarkers for the clinical pattern identification of CHD-PAF patients with heart-spleen yang deficiency and stagnant phlegm syndrome.

关 键 词：阵发性心房颤动 冠心病 痰浊证 胆汁酸代谢组 生物标志物 

分 类 号：R259[医药卫生—中西医结合]