Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1  

作　　者：Dong Liu Fan Jia Ya Wei Yuye Li Shuyun Meng Tianyan You 

机构地区：[1]Key Laboratory of Modern Agricultural Equipment and Technology,Ministry of Education,School of Agricultural Engineering,Jiangsu University,Zhenjiang,Jiangsu,212013 China

出　　处：《Chinese Journal of Chemistry》2022年第18期2232-2238,共7页中国化学（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.61901193,22074055);the Natural Science Foundation of Jiangsu Province(No.BK20200104);the Innovation/Entrepreneurship Program of Jiangsu Province,the Project of Faculty of Agricultural Equipment of Jiangsu University,and Priority Academic Program Development of Jiangsu Higher Education Institutions.

摘　　要：Ratiometric biosensor is among the promising strategies for the advanced analysis;its applicability in multiple scenarios,however,is seriously limited for the fixed analytical feature.Here,we reported ratiometric electrochemical biosensors with programmable analytical feature by alternating the binding site of ferrocene(Fc)to DNA duplex for the detection of aflatoxin B1(AFB1).DNA duplex was synthesized with AFB1 aptamer and its complementary DNA(cDNA)with Fc molecular tagged on aptamer or cDNA.For analysis,aptamer-AFB1 complex stripped from the electrode,and free cDNA triggered hybridization chain reaction to produce double-stranded DNA for methylene blue(MB)absorption.In this way,redox currents of Fc(I_(Fc))and absorbed MB(I_(MB))at the electrode were collected to produce the ratiometric signal of I_(MB)/I_(Fc)as a yardstick for AFB1.When Fc was tagged on aptamer,IFc decreased with higher concentration of AFB1(C_(AFB1)),the resultant biosensor allowed detection with an On/Off(I_(MB)/I_(Fc))mode;when Fc was tagged on cDNA,I_(Fc) kept constant as C_(AFB1)varying,offering an On/Constant(I_(MB)/I_(Fc))mode for the detection.Consequently,biosensor with On/Off mode showed a linear range of 0.1—100 pg/mL with a limit of detection(LOD)of 0.0650 pg/mL,while biosensor with On/Constant mode offered a wider linear rang of 1—10000 pg/mL but a relatively poor LOD of 0.330 pg/mL.Our work revealed the vital importance of binding site of probes to DNA,and this principle can endow ratiometric biosensor with programmable analytical feature(e.g.,linear range,LOD),allowing detections in various scenarios.

关 键 词：ELECTROCHEMISTRY Biosensors Aptamers Ratiometric detection AFLATOXINS 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]