健脾活血方对肝硬化脾功能亢进兔脾切除后血瘀证的影响  

Effect of Jianpi Huoxue Recipe(健脾活血方)on Blood Stasis Syndrome after Splenectomy in Rabbits with Hypersplenism due to Cirrhosis

作　　者：黄龙[1,2] 于庆生 彭辉[1] HUANG Long;YU Qingsheng;PENG Hui(The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei Anhui 230031,China;Institute of Traditional Chinese Medicine Surgery,Anhui Academy of Traditional Chinese Medicine,Hefei Anhui 230031,China)

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031 [2]安徽省中医药科学院中医外科研究所,安徽合肥230031

出　　处：《中医药导报》2022年第12期5-10,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：安徽省自然科学基金项目(81573987)。

摘　　要：目的:研究健脾活血方对硫代乙酰胺(TAA)诱导的肝硬化脾功能亢进兔脾切除术后血瘀证的影响。方法:选择月龄为3个月新西兰大白兔,取60只随机分成对照组、肝硬化模型组、肝硬化脾切除组、健脾活血方低剂量组、健脾活血方中剂量组和健脾活血方高剂量组,每组10只。除对照组外,其余各组兔采用TAA诱导肝硬化脾功能亢进模型。造模成功后,肝硬化脾切除组和健脾活血方低、中、高剂量组兔制备肝硬化脾切除模型。各药物组兔每天于09∶00∶00和15∶00∶00灌胃给予相应药物,肝硬化脾切除组、肝硬化模型组和对照组灌胃给予生理盐水,2次/d,连续灌胃2周。检测各组兔血瘀证指标[血栓素B_(2)(TXB_(2))、6-酮-前列腺素F_(1α)(6-keto-PGF_(1α))、内皮素-1(ET-1)、一氧化氮(NO)、组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制物(PAI)]、凝血指标[凝血酶原时间(PT)、纤维蛋白原(FIB)、D-二聚体(DD)]、血液流变学指标(高切黏度、中切黏度、低切黏度、血浆黏度、红细胞压积、红细胞变形指数、刚性指数)、血常规[血小板计数、平均血小板体积(MPV)、血小板分布宽度(PDW)、血小板压积(PCT)]及血小板膜蛋白CD63、CD62p的表达情况;HE染色观察肝脾病理学变化。结果:肝硬化模型组兔肝小叶结构消失,且形成大小不等假小叶,存在炎症细胞浸润和胆管细胞增生。与对照组比较,肝硬化模型组兔TXB_(2)、6-keto-PGF_(1α)、ET-1、NO、t-PA和PAI均升高,PT延长,FIB降低,DD升高,高切黏度、中切黏度、低切黏度及血浆黏度均升高,血小板计数、MPV、PDW、PCT均降低,血小板膜蛋白CD63、CD62p均升高,差异均有统计学意义(P<0.05)。肝硬化脾切除组兔肝小叶结构消失,肝细胞水肿至气球样变性,肝窦扩张淤血。与肝硬化模型组比较肝硬化脾切除组兔6-Keto-PGF_(1α)、TXB_(2)均升高,NO浓度、PAI、t-PA、ET-1均降低,PT缩短,FIB、DDObjective:To study the effect of Jianpi Huoxue Recipe(健脾活血方,JPHXR)on blood stasis syndrome after splenectomy in rabbits with liver cirrhosis and hypersplenism induced by thioacetamide(TAA).Methods:60 New Zealand white rabbits aged 3 months were randomly divided into control group,liver cirrhosis model group,liver cirrhosis splenectomy group,low dose JPHXR group,middle dose JPHXR group and high dose JPHXR group,with 10 rabbits in each group.In addition to the control group,the other groups of rabbits were treated with TAA induced hypersplenism model of liver cirrhosis.After the model was established successfully,the liver cirrhosis splenectomy group and the low,middle and high dose groups of JPHXR were used to prepare the liver cirrhosis splenectomy model.The rabbits in each drug group were given corresponding drugs by gavage at 09∶00∶00 and 15∶00∶00 every day,and the liver cirrhosis splenectomy group,liver cirrhosis model group and control group were given normal saline by gavage twice a day for two consecutive weeks.The indexes of blood stasis syndrome[content of thromboxane B_(2)(TXB_(2)),6-keto-prostaglandin F_(1α)(6-keto-PGF_(1α)),endothelin-1(ET-1),nitric oxide(NO),tissue plasminogen activator(t-PA)and plasminogen activator inhibitor(PAI)],blood coagulation indexes[prothrombin time(PT),fibrinogen(FIB),D-dimer(DD)],blood rheology indexes[high,middle and low shear viscosity,plasma viscosity,hematocrit,erythrocyte deformation index and rigidity index],blood routine examination[platelet count,mean platelet volume(MPV),platelet distribution width(PDW)and platelet hematocrit(PCT)]and the expression of platelet membrane proteins CD63 and CD62p were monitored in each group.HE staining was used to observe the pathological changes of the liver and spleen.Results:In liver cirrhosis model group,the structure of hepatic lobule disappeared,and pseudolobules of different sizes were formed,with inflammatory cell infiltration and bile duct cell proliferation.Compared with the control group,TXB_(2),6-keto-

关 键 词：肝硬化 脾切除术 血瘀证 健脾活血方 兔 

分 类 号：R285.5[医药卫生—中药学]