磁共振3D DANTE CUBE序列对动脉管壁的定量评估:与病理比较的实验研究  

Quantitative assessment of arterial wall by magnetic resonance 3D DANTE CUBE sequence:An experimental study compared with pathology

作　　者：赵海燕 陈玉坤 陈录广 蒋慧 陈士跃 李帅 彭雯佳 陆建平 ZHAO Haiyan;CHEN Yukun;CHEN Luguang;JIANG Hui;CHEN Shiyue;LI Shuai;PENG Wenjia;LU Jianping(Department of Radiology,the First Affiliated Hospital of PLA Naval Medical University,Shanghai 200433,China;Department of Pathology,the First Affiliated Hospital of PLA Naval Medical University,Shanghai 200433,China)

机构地区：[1]中国人民解放军海军军医大学第一附属医院放射诊断科,上海200433 [2]中国人民解放军海军军医大学第一附属医院病理科,上海200433

出　　处：《磁共振成像》2022年第12期111-116,123,共7页Chinese Journal of Magnetic Resonance Imaging

基　　金：上海市自然科学基金(编号:22ZR1478100、20ZR1456300);上海市科委医学创新研究专项项目(编号:22Y11911200)。

摘　　要：目的定量评价基于延迟交变章动调整激发(delay alternating with nutation for tailored excitation,DANTE)预脉冲3D CUBE磁共振序列对动脉管壁成像与病理结果的一致性。材料与方法采用高脂饲料喂养加腹主动脉内膜拉伤手术制作12只新西兰大白兔的动脉粥样硬化模型,同期普通饲料喂养8只作为对照组。运用3.0 T磁共振扫描仪对腹主动脉行冠状位黑血DANTE预备的CUBE容积扫描,包括T1WI和T2WI序列,而后行病理HE染色及高清数字化扫描。对磁共振图像进行横轴位重建,测量磁共振和病理图像管腔面积(lumen area,LA)、血管总面积(total area,TA),计算管壁面积(wall area,WA),WA=TA-LA,以及计算标准化管壁指数(normalized wall index,NWI),NWI=WA/TA。T1WI和T2WI的NWI分别用NWI_(T1)和NWI_(T2)表示。根据病理图片勾画血管壁外膜内边缘和外膜外边缘,相应的NWI分别用NWI_(P1)和NWI_(P2)表示。比较影像和病理定量指标在实验组与对照组之间的差异,并采用组内相关系数(intraclass correlation coefficient,ICC)、Bland-Altman plots评估磁共振序列之间、磁共振与病理之间的NWI一致性。结果最终19只兔(实验组1只磁共振成像检查前死亡)纳入统计。与对照组相比,实验组的LA明显较小(T1WI,P=0.033;T2WI,P<0.0001)、NWI明显较大(T1WI,P<0.0001;T2WI,P<0.0001)。整体一致性分析发现,NWI_(T1)和NWI_(T2)之间的一致性较好(ICC=0.7552),NWI_(T1)或NWI_(T2)与NWI_(P1)的一致性次之(ICC=0.438;ICC=0.444),NWI_(T1)或NWI_(T2)与NWI_(P2)的一致性最差(ICC=0.285;ICC=0.292)。实验组的一致性分析与上述结果类似。对照组中,NWI_(T1)和NWI_(T2)之间的一致性也较好(ICC=0.6304),但NWI_(T1)或NWI_(T2)与NWI_(P1)的一致性较差(ICC=-0.1917;ICC=-0.05949),NWI_(T1)或NWI_(T2)与NWI_(P2)的一致性也较差(ICC=-0.1989;ICC=-0.1441)。结论3D DANTE CUBE的T1WI和T2WI可以量化区分兔腹主动脉粥样硬化病变管壁与正常管壁,且这两个常用加权序列之间的一�Objective:To quantitatively evaluate the consistency of arterial wall imaging and pathological results based on DANTE prepulse 3D CUBE magnetic resonance sequence.Materials and Methods:Twelve New Zealand white rabbits were fed high-fat diet plus abdominal aortic strain surgery to establish atherosclerosis model,and 8 rabbits were fed ordinary diet as control group.The abdominal aorta was scanned with coronal black-blood DANTE CUBE T1WI and T2WI sequences using 3.0 T MRI system,followed by pathological HE staining.The MRI images were reconstructed in the horizontal axis,and lumen area(LA)and total area(TA)were measured in MRI and pathological images.The wall area(WA)was calculated,WA=TA-LA,and the normalized wall index(NWI)was calculated,NWI=WA/TA.The NWI of T1WI and T2WI are denoted by NWI_(T1)and NWI_(T2),respectively.The inner edge and outer edge of the adventitial of the vessel wall were delineated according to the pathological images,and the corresponding NWI were denoted by NWI_(P1)and NWI_(P2),respectively.The differences of MR imaging and pathological quantitative indexes between the experimental group and the control group were compared,and the NWI consistency between MRI sequences and between MRI and pathology were evaluated by intraclass correlation coefficient(ICC)and Bland-Altman plots.Results:Finally,19 rabbits(1 died before MRI examination in the experimental group)were included in the statistics.Compared with the control group,the LA of the experimental group was significantly smaller(T1WI,P=0.033;T2WI,P<0.0001)and NWI were significantly larger(T1WI,P<0.0001;T2WI,P<0.0001).The overall consistency analysis showed that the consistency between NWI_(T1)and NWI_(T2)was better(ICC=0.7552),followed by the consistency between NWI_(T1)or NWI_(T2)and NWI_(P1)(ICC=0.438;ICC=0.444),NWI_(T1)or NWI_(T2)had the worst agreement with NWI_(P2)(ICC=0.285;ICC=0.292).The consistency analysis of the experimental group was similar to the above results.In the control group,the agreement between NWI_(T1)and NWI_(T2)was acc

关 键 词：磁共振成像 血管壁 动脉粥样硬化 兔 斑块 黑血成像 延迟交变章动调整激发 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]