不同倍性紫果西番莲“满天星”形态、叶绿素及荧光参数差异比较  

Comparison of Morphology,Chlorophyll and Chlorophyll Fluorescence Parameters of Passiflora edulis with Different Ploidy

作　　者：王雪[1,2] 朱雅静 陈诗[1,2] 杨振欣 罗茜[1,2] 许玉兰[1,2] 王德新[1,2] 蔡年辉[1,2] WANG Xue;ZHU Ya-jing;CHEN Shi;YANG Zheng-xin;LUO Xi;XU Yu-lan;WANG De-xin;CAI Nian-hui(Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Southwest Forestry University,Ministry of Education,Kunming 650224,China;Key Laboratory of biodiversity conservation in Southwest China,State Forestry Administration,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明650224 [2]西南林业大学西南地区生物多样性保育国家林业局重点实验室,云南昆明650224

出　　处：《山东农业大学学报（自然科学版）》2022年第5期755-762,共8页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-075);云南省研究生导师团队建设项目(2022-97);西南林业大学第一批预研基金(01108-18200133)。

摘　　要：为了探寻两个倍性植株的形态、叶绿素及叶绿素荧光参数的差异,揭示不同倍性光合能力的变异规律,本研究以二倍体和三倍体的紫果西番莲“满天星”(Passiflora edulis)的苗木叶片为材料,测定植株形态及叶绿素含量,用高速连续激发式荧光仪Handy PEA测定其叶绿素荧光参数及叶绿素荧光诱导动力学曲线。结果表明,三倍体植株表现出矮壮、叶柄更长、叶片更宽的现象,三倍体较二倍体叶绿素a、叶绿素b、叶绿素总含量、类胡萝卜素含量大,三倍体对环境的光照辐射适应性较好、不易受到光抑制,光合速率更高。在电子传递过程中,三倍体比二倍体变化波动小,量子比率和热耗散的量子比率方面的分配比较稳定,PSⅡ结构的能量传递较稳定,适应性更强。在不同倍性植株中,二倍体和三倍体的OJIP一致,三倍体的荧光强度明显高于二倍体,说明三倍体的荧光产量更高,接受光量子的能量更好,因此光合碳代谢速率更高。In order to explore the difference of morphology,chlorophyll and chlorophyll fluorescence parameters between two ploidy plants and reveal the variation rule of different ploidy photosynthetic capacity,this study used the seedling leaves of two ploidy Passiflora edulis"Mantianxing"as materials to determine the plant morphology and chlorophyll content,and measured the chlorophyll fluorescence parameters and chlorophyll fluorescence induction kinetics curve with the high-speed continuous excitation fluorescence meter Handy PEA.The results showed that triploid plants were short and strong,with longer petioles and wider leaves.Compared with diploid plants,triploid plants had more chlorophyll a,chlorophyll b,total chlorophyll content and carotenoid content.Triploid plants had better adaptability to light and radiation,were not easily inhibited by light,and had higher photosynthetic rate.In the process of electron transfer,triploid has less fluctuation than diploid,the distribution of quantum ratio and heat dissipation quantum ratio is relatively stable,and the energy transfer of PSⅡstructure is relatively stable and more adaptive.In different ploidy plants,the OJIP of diploid and triploid is the same,and the fluorescence intensity of triploid is significantly higher than that of diploid,indicating that triploid has higher fluorescence yield,better energy to receive light quanta,and therefore higher photosynthetic carbon metabolism rate.

关 键 词：紫果西番莲 生物学 叶绿素 荧光 

分 类 号：S667.9[农业科学—果树学]